ArticleCurrent protocols in stem cell biology2019
An Improved Two-Step Protocol for Trophoblast Differentiation of Human Pluripotent Stem Cells.
Article in Current protocols in stem cell biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- BMP4 and FGF2 promote heterogeneous priming including extraembryonic lineages in porcine expanded potential stem cells.Frontiers in cell and developmental biology · 2026Article
- VGLL1 contributes to both the transcriptome and epigenome of the developing trophoblast compartment.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Chromosomal instability in human trophoblast stem cells and placentas.Nature communications · 2025Article
- The modeling of human implantation and early placentation: achievements and perspectives.Human reproduction update · 2025Review
- Modeling human placental biology: a review of organoid technologies.Frontiers in cell and developmental biology · 2025Review
- Autophagy in reproduction and pregnancy-associated diseases.iScience · 2024Review
- Rapid retinoic acid-induced trophoblast cell model from human induced pluripotent stem cells.Scientific reports · 2024Article
- TGFβ signalling: a nexus between inflammation, placental health and preeclampsia throughout pregnancy.Human reproduction update · 2024Review
- iPSC-based modeling of preeclampsia identifies epigenetic defects in extravillous trophoblast differentiation.iScience · 2024Article
- Human archetypal pluripotent stem cells differentiate into trophoblast stem cells via endogenous BMP5/7 induction without transitioning through naive state.Scientific reports · 2024Article
- Human fetal tissue is critical for biomedical research.Stem cell reports · 2023Review
- Current Strategies of Modeling Human Trophoblast Using Human Pluripotent Stem Cells in vitro.Current protocols · 2023Article
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- Article
- Exploring maternal-fetal interface with in vitro placental and trophoblastic models.Frontiers in cell and developmental biology · 2023Review
- The role of BMP4 signaling in trophoblast emergence from pluripotency.Cellular and molecular life sciences : CMLS · 2022Review
- Derivation of functional trophoblast stem cells from primed human pluripotent stem cells.Stem cell reports · 2022Article
- The product of BMP-directed differentiation protocols for human primed pluripotent stem cells is placental trophoblast and not amnion.Stem cell reports · 2022Article
- Review
- Role of autocrine bone morphogenetic protein signaling in trophoblast stem cells†.Biology of reproduction · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We previously established a two-step protocol for differentiation of human pluripotent stem cells (hPSCs) into trophoblasts, using a StemPro-based minimal medium (EMIM) with bone morphogenetic protein-4 (BMP4). This protocol was suboptimal, resulting in induction of mixed mesoderm and trophoblast markers. Furthermore, adapting hPSCs to StemPro has proven difficult, and prolonged culture in this medium has been shown to promote genomic instability. Therefore, we moved on to the use of new media, including E8, and most recently, StemFlex, for rapid adaptation from feeder to non-feeder conditions. In the new protocol, we have incorporated the WNT inhibitor IWP2 into the first step, resulting in uniform differentiation of hPSCs into cytotrophoblast (CTB)-like cells, without induction of the mesoderm lineage. We also show that, at the end of the second step, there are distinct populations of terminally differentiated multinucleated human chorionic gonadotropin (hCG)-producing syncytiotrophoblast (STB) and HLAG
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.