ArticlePLoS pathogens2019
Molecular basis for functional diversity among microbial Nep1-like proteins.
Article in PLoS pathogens, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Transcription Factor PFB1 Is Required for the Botrytis cinerea Effector BcSCR1-Mediated Pathogenesis.Molecular plant pathology · 2026Article
- Plasticity of the cytotoxic Nep1-like protein enables promiscuity in binding to its lipid receptor glycosylinositol phosphorylceramides.Science advances · 2025Article
- SsNEP2 Plays a Role in the Interaction BetweenJournal of fungi (Basel, Switzerland) · 2025Article
- Volatile Formulation ofIndian journal of microbiology · 2024Article
- Disruption of plant plasma membrane by Nep1-like proteins in pathogen-plant interactions.The New phytologist · 2023Review
- Characteristics, Roles and Applications of Proteinaceous Elicitors from Pathogens in Plant Immunity.Life (Basel, Switzerland) · 2023Review
- Advances in Fungal Elicitor-Triggered Plant Immunity.International journal of molecular sciences · 2022Review
- Knowing me, knowing you: Self and non-self recognition in plant immunity.Essays in biochemistry · 2022Article
- A secreted ribonuclease effector from Verticillium dahliae localizes in the plant nucleus to modulate host immunity.Molecular plant pathology · 2022Article
- Cytotoxic activity of Nep1-like proteins on monocots.The New phytologist · 2022Article
- Unraveling Plant Cell Death duringMicroorganisms · 2022Review
- Article
- An oomycete NLP cytolysin forms transient small pores in lipid membranes.Science advances · 2022Article
- Characterization and necrosis-inducing activity of necrosis- and ethylene-inducing peptide 1-like proteins fromFrontiers in microbiology · 2022Article
- Nep1-Like Proteins From the Biocontrol AgentFrontiers in plant science · 2022Article
- The Botrytis cinerea Crh1 transglycosylase is a cytoplasmic effector triggering plant cell death and defense response.Nature communications · 2021Article
- Nep1-like proteins as a target for plant pathogen control.PLoS pathogens · 2021Article
- Nitrogen Supply and Host-Plant Genotype Modulate the Transcriptomic Profile ofFrontiers in microbiology · 2021Article
- Devastating intimacy: the cell biology of plant-Phytophthora interactions.The New phytologist · 2020Review
- Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Necrosis and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) are secreted by several phytopathogenic microorganisms. They trigger necrosis in various eudicot plants upon binding to plant sphingolipid glycosylinositol phosphorylceramides (GIPC). Interestingly, HaNLP3 from the obligate biotroph oomycete Hyaloperonospora arabidopsidis does not induce necrosis. We determined the crystal structure of HaNLP3 and showed that it adopts the NLP fold. However, the conformations of the loops surrounding the GIPC headgroup-binding cavity differ from those of cytotoxic Pythium aphanidermatum NLPPya. Essential dynamics extracted from μs-long molecular dynamics (MD) simulations reveals a limited conformational plasticity of the GIPC-binding cavity in HaNLP3 relative to toxic NLPs. This likely precludes HaNLP3 binding to GIPCs, which is the underlying reason for the lack of toxicity. This study reveals that mutations at key protein regions cause a switch between non-toxic and toxic phenotypes within the same protein scaffold. Altogether, these data provide evidence that protein flexibility is a distinguishing trait of toxic NLPs and highlight structural determinants for a potential functional diversification of non-toxic NLPs utilized by biotrophic plant pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.