ArticleJournal of visualized experiments : JoVE2019
Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions.
Article in Journal of visualized experiments : JoVE, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Simplified Anaerobic Cultivation ofBioengineering (Basel, Switzerland) · 2026Article
- Control of alluvial aquifer architecture on reductive and oxidative dechlorination of chloroethenes.Environmental science and pollution research international · 2026Article
- Unlocking Microbial Dark Matter: A Comprehensive Review of Isolation Technologies from Traditional Culturing to Single-Cell Technologies.Microorganisms · 2026Review
- Environmental selection constrains metabolic network architecture despite taxonomic turnover in anaerobic digestion communities.The ISME journal · 2026Article
- Laminin functionalization of zirconia abutments modulates early oral biofilm formation in vitro.Journal of oral microbiology · 2026Article
- Optimizing dark fermentation for hydrogen production: lessons fromFrontiers in microbiology · 2026Article
- Comparative phenotypic and genomic analysis of the methanogen Methanomethylovorans thermophila L2FAW and its phylogenomic placement within the Genome Taxonomy Database.Access microbiology · 2026Article
- Preserving fermented-foods microbial diversity through systematic culturomics for the discovery of multi-strain probiotic candidates.Current research in food science · 2026Article
- Phenyl Acid Induced Inhibition of Methanogenesis in COEnvironmental microbiology reports · 2025Article
- Shaken not stirred - effect of different mixing modes during the cultivation of methanogenic pure cultures.Current research in microbial sciences · 2025Article
- Advances in gut microbiota functions in inflammatory bowel disease: Dysbiosis, management, cytotoxicity assessment, and therapeutic perspectives.Computational and structural biotechnology journal · 2025Review
- Draft genome sequence ofMicrobiology resource announcements · 2024Article
- Pre-treatment with Trichoderma viride: Towards a better understanding of its consequences for anaerobic digestion.Environmental microbiology reports · 2024Article
- Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins.Nature protocols · 2024Review
- Gasification chars and activated carbon: Systematic physico-chemical characterization and effect on biogas production.Heliyon · 2024Article
- Ferriphaselus amnicola strain GF-20, a new iron- and thiosulfate-oxidizing bacterium isolated from a hard rock aquifer.FEMS microbiology ecology · 2024Article
- Meso- and thermophilic posttreatment of press water coming from a thermophilic municipal solid waste digester.Biotechnology and bioengineering · 2024Article
- Article
- Biofilm formation and cell plasticity drive diazotrophy in an anoxygenic phototrophic bacterium.Applied and environmental microbiology · 2023Article
- Effects of phenyl acids on different degradation phases during thermophilic anaerobic digestion.Frontiers in microbiology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
In contrast to aerobic organisms, strictly anaerobic microorganisms require the absence of oxygen and usually a low redox potential to initiate growth. As oxygen is ubiquitous in air, retaining O2-free conditions during all steps of cultivation is challenging but a prerequisite for anaerobic culturing. The protocol presented here demonstrates the successful cultivation of an anaerobic mixed culture derived from a biogas plant using a simple and inexpensive method. A precise description of the entire anoxic culturing process is given including media preparation, filling of cultivation flasks, supplementation with redox indicator and reducing agents to provide low redox potentials as well as exchanging the headspace to keep media free from oxygen. Furthermore, a detailed overview of aseptically inoculating gas tight serum flasks (by using sterile syringes and needles) and suitable incubation conditions is provided. The present protocol further deals with gas and liquid sampling for subsequent analyses regarding gas composition and volatile fatty acid concentrations using gas chromatography (GC) and high performance liquid chromatography (HPLC), respectively, and the calculation of biogas and methane yield considering the ideal gas law.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.