ArticleFrontiers in bioengineering and biotechnology2019
Investigating Fibroblast-Induced Collagen Gel Contraction Using a Dynamic Microscale Platform.
Article in Frontiers in bioengineering and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Trial
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- Comparative evaluation of 3D culture strategies for pulp-dentin models.BMC oral health · 2026Article
- Reinforcement of Fibrillar Collagen Hydrogels with Bioorthogonal Covalent Crosslinks.Biomacromolecules · 2025Article
- In situ UNIversal Orthogonal Network (UNION) bioink deposition for direct delivery of corneal stromal stem cells to corneal wounds.Bioactive materials · 2025Article
- The Development of 3D Primary Co-Culture Models of the Human Airway.International journal of molecular sciences · 2025Article
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- Interpenetrating networks of fibrillar and amorphous collagen promote cell spreading and hydrogel stability.Acta biomaterialia · 2025Article
- Embedded 3D Bioprinting of Collagen Inks into Microgel Baths to Control Hydrogel Microstructure and Cell Spreading.Advanced healthcare materials · 2024Article
- Assessment of Tilapia Skin Collagen for Biomedical Research Applications in Comparison with Mammalian Collagen.Molecules (Basel, Switzerland) · 2024Article
- Engineering large and geometrically controlled vascularized nerve tissue in collagen hydrogels to restore large-sized volumetric muscle loss.Biomaterials · 2023Article
- Tissue-Engineered Cochlear Fibrosis Model Links Complex Impedance to Fibrosis Formation for Cochlear Implant Patients.Advanced healthcare materials · 2023Article
- A roadmap for developing and engineeringBiophysics reviews · 2023Review
- Article
- In vitro bioreactor for mechanical control and characterization of tissue constructs.Journal of biomechanics · 2023Article
- Vascularization in Bioartificial Parenchymal Tissue: Bioink and Bioprinting Strategies.International journal of molecular sciences · 2022Review
- Engineering a 3D In Vitro Model of Human Gingival Tissue Equivalent with Genipin/Cytochalasin D.International journal of molecular sciences · 2022Article
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Authors and funding
9 authors.
Funding
Abstract
Mechanical forces have long been recognized as fundamental drivers in biological processes, such as embryogenesis, tissue formation and disease regulation. The collagen gel contraction (CGC) assay has served as a classic tool in the field of mechanobiology to study cell-induced contraction of extracellular matrix (ECM), which plays an important role in inflammation and wound healing. In a conventional CGC assay, cell-laden collagen is loaded into a cell culture vessel (typically a well plate) and forms a disk-shaped gel adhering to the bottom of the vessel. The decrement in diameter or surface area of the gel is used as a parameter to quantify the degree of cell contractility. In this study, we developed a microscale CGC assay with an engineered well plate insert that uses surface tension forces to load and manipulate small volumes (14 μL) of cell-laden collagen. The system is easily operated with two pipetting steps and the microscale device moves dynamically as a result of cellular forces. We used a straightforward one-dimensional measurement as the gel contraction readout. We adapted a conventional lung fibroblast CGC assay to demonstrate the functionality of the device, observing significantly more gel contraction when human lung fibroblasts were cultured in serum-containing media vs. serum-free media (
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.