ArticleFrontiers in microbiology2019
A Robust and Universal Metaproteomics Workflow for Research Studies and Routine Diagnostics Within 24 h Using Phenol Extraction, FASP Digest, and the MetaProteomeAnalyzer.
Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed.
- Multi-omics characterization of β-myrcene-evolvedFrontiers in molecular biosciences · 2026Article
- Proteomic Insights into Bacterial Responses to Antibiotics: A Narrative Review.International journal of molecular sciences · 2025Review
- The microbiologist's guide to metaproteomics.iMeta · 2025Review
- Metaproteomics in the One Health framework for unraveling microbial effectors in microbiomes.Microbiome · 2025Review
- Metaproteomic Analysis of Fermented Vegetable Formulations with Lactic Acid Bacteria: A Comparative Study from Initial Stage to 15 Days of Production.Foods (Basel, Switzerland) · 2025Article
- Quantification of intracellular influenza A virus protein dynamics in different host cells after seed virus adaptation.Applied microbiology and biotechnology · 2025Article
- Quantitative analysis of proteomic changes in two monoclonal suspension MDCK cell lines infected with human influenza A virus (H1N1).PloS one · 2025Article
- metaExpertPro: A Computational Workflow for Metaproteomics Spectral Library Construction and Data-Independent Acquisition Mass Spectrometry Data Analysis.Molecular & cellular proteomics : MCP · 2024Article
- Benchmarking low- and high-throughput protein cleanup and digestion methods for human fecal metaproteomics.mSystems · 2024Article
- Detection, isolation and characterization of phage-host complexes using BONCAT and click chemistry.Frontiers in microbiology · 2024Article
- Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesized proteins.ISME communications · 2024Article
- Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Metaproteomics to understand how microbiota function: The crystal ball predicts a promising future.Environmental microbiology · 2023Article
- Adaptation of a microbial community to demand-oriented biological methanation.Biotechnology for biofuels and bioproducts · 2022Article
- Rapid Proteomic Characterization of Bacteriocin-ProducingInternational journal of molecular sciences · 2022Article
- Towards standardized and reproducible research in skin microbiomes.Environmental microbiology · 2022Review
- Discovering Biomarkers for Non-Alcoholic Steatohepatitis Patients with and without Hepatocellular Carcinoma Using Fecal Metaproteomics.International journal of molecular sciences · 2022Article
- Associated bacterial microbiome responds opportunistic once algal host Scenedesmus vacuolatus is attacked by endoparasite Amoeboaphelidium protococcarum.Scientific reports · 2022Article
- Does filter-aided sample preparation provide sufficient method linearity for quantitative plant shotgun proteomics?Frontiers in plant science · 2022Article
- Metaomics in Clinical Laboratory: Potential Driving Force for Innovative Disease Diagnosis.Frontiers in microbiology · 2022Review
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The investigation of microbial proteins by mass spectrometry (metaproteomics) is a key technology for simultaneously assessing the taxonomic composition and the functionality of microbial communities in medical, environmental, and biotechnological applications. We present an improved metaproteomics workflow using an updated sample preparation and a new version of the MetaProteomeAnalyzer software for data analysis. High resolution by multidimensional separation (GeLC, MudPIT) was sacrificed to aim at fast analysis of a broad range of different samples in less than 24 h. The improved workflow generated at least two times as many protein identifications than our previous workflow, and a drastic increase of taxonomic and functional annotations. Improvements of all aspects of the workflow, particularly the speed, are first steps toward potential routine clinical diagnostics (i.e., fecal samples) and analysis of technical and environmental samples. The MetaProteomeAnalyzer is provided to the scientific community as a central remote server solution at www.mpa.ovgu.de.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.