ArticleMikrochimica acta2019
Catalytic hairpin assembly-assisted lateral flow assay for visual determination of microRNA-21 using gold nanoparticles.
Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- New Frontiers for the Early Diagnosis of Cancer: Screening miRNAs Through the Lateral Flow Assay Method.Biosensors · 2025Review
- Rapid Detection of microRNA-122 in Serum and Finger Blood Using a Lateral Flow Nucleic Acid Biosensor.Biosensors · 2025Article
- Addition of dNTPs can improve the detection sensitivity of catalytic hairpin assembly.iScience · 2023Article
- Role of Nano-miRNAs in Diagnostics and Therapeutics.International journal of molecular sciences · 2022Review
- Au@Ag core-shell nanoparticles for microRNA-21 determination based on duplex-specific nuclease signal amplification and surface-enhanced Raman scattering.Mikrochimica acta · 2020Article
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Authors and funding
6 authors.
Funding
Abstract
The authors describe an improved lateral flow assay based on (a) the use of catalytic hairpin assembly (CHA), and (b) on signal amplification performed at the interface of gold nanoparticles (AuNPs). The combination of the amplification capability of the CHA reaction and the unique optical properties of AuNPs results in an assay that has a sensitivity that is improved by more than two orders of magnitude. MicroRNA-21 was employed as a model analyte to prove the concept. The presence of microRNA-21 triggers the self-assembly of two hairpin DNAs into double stranded DNA and exposing biotin molecules on the surface of AuNPs. Hence, the target becomes recycled and the signal is strongly amplified. The AuNPs carrying biotin are captured on the test line of the strip to display a red zone. This enables the visual recognition of microRNA without the need for any instrumentation. The fast quantitation of microRNA via the red band intensity is accomplished with the help of software, and the limit of detection is 0.89 pM. The enhanced lateral flow assay was employed to the determination of microRNA-21 in cell extracts and spiked serum samples. Graphical abstract A lateral flow assay for microRNA is described with a detection limit that is improved by two orders of magnitude. It is based on catalytic hairpin assembly (CHA) signal amplification performed at the interface of gold nanoparticles.
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Registered trials
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