Evidence map›Paper›PMID 31471765›Full record

ArticleMikrochimica acta2019

Electrochemical thrombin aptasensor based on using magnetic nanoparticles and porous carbon prepared by carbonization of a zinc(II)-2-methylimidazole metal-organic framework.

Qian Ren, Xianzhen Xu, Guangping Cao, Jianfei Xia, Zonghua Wang, Qingyun Liu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

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3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Qian RenCollege of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, the Growing Base for State Key Laboratory, Qingdao University, Qingdao, 266071, People's Republic of China.
Xianzhen XuCollege of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, the Growing Base for State Key Laboratory, Qingdao University, Qingdao, 266071, People's Republic of China.
Guangping CaoCollege of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, the Growing Base for State Key Laboratory, Qingdao University, Qingdao, 266071, People's Republic of China.
Jianfei XiaCollege of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, the Growing Base for State Key Laboratory, Qingdao University, Qingdao, 266071, People's Republic of China. xiajianfei@126.com.ORCID 0000-0001-8555-9230
Zonghua WangCollege of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, the Growing Base for State Key Laboratory, Qingdao University, Qingdao, 266071, People's Republic of China.
Qingyun LiuCollege of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao, People's Republic of China.

Funding

National Natural Science Foundation of China (CN) 21645007Natural Science Foundation of Shandong Province (CN) ZR2016BM21Taishan Scholar Foundation of Shandong Province ts201511027
6 · The paper itself

Abstract

A homogeneous electrochemical aptasensor was obtained by modifying a glassy carbon electrode (GCE) with a porous carbon nanomaterial (Z-1000, about 70 nm, deteced by transmission electron microscopic) that was obtained by carbonization of a zinc(II)-2-methylimidazole metal-organic framework. Z-1000 possesses a large specific surface and outstanding electrochemical properties. A thrombin-binding aptamer (CP) was immobilized on the magnetite nanoparticles MNPs by the condensation reaction and further combined with reporter probe (RP) that is functionalized with electroactive methylene blue (MB). In the presence of thrombin, the CP was specifically recognized with it to form the CP/MNP/Thb complex, and the RP was dissociated from MNPs. The released RP was captured by the modified GCE through π-stacking interaction between nucleobases and carbon nanostructure. The electrical signal generated by MB can be monitored by differential pulse voltammetry (DPV). Under the optimized conditions, the DPV peak current at around -0.28 V (vs. SCE) increases with thrombin concentration. The sensor has a detection limit of 0.8 fM of thrombin and a linear range that extends from 10 fM to 100 nM. It was successfully applied to the analysis of spiked serum. The recoveries are 98.1-99.4% and RSDs are 3.9%-4.0%. Conceivably, this aptasensor scheme can be easily extended to other proteins and gives inspiration to manufacture sensitive aptasensor. Graphical abstract A homogeneous electrochemical aptasensor is obtained by modifying a glassy carbon electrode with the MOF-derived porous carbon. The sensor has a detection limit of 0.8 fM and a wide linear range from 10 fM to 100 nM for thrombin detection.

Indexed as

Electrochemical biosensorMagnetic nanomaterialPorous carbon nanostructureProtein detectionZeolitic imidazolate framework

Identifiers

PMID31471765

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