ArticleScientific reports2019
Transcriptomic and functional analyses of 3D placental extravillous trophoblast spheroids.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Recapitulating gestational progression: a high-throughput 3D bioprinted vascularized human placenta demonstrating stage-specific molecular and functional signatures of human pregnancy.Scientific reports · 2026Article
- Article
- Brain endothelial spheroids and cortical organoids reveal the impact of Toxoplasma gondii lineage and host-phagocyte-pathogen interactions on colonization.Cellular and molecular life sciences : CMLS · 2026Article
- Beyond monolayers: a comparative analysis of 2D cell cultures and 3DFrontiers in bioengineering and biotechnology · 2026Review
- Biophysical Considerations in Cell Fusion.Advances in experimental medicine and biology · 2026Review
- Real-Time Visualization of Endogenous HChemical & biomedical imaging · 2025Article
- A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Novel 3D human trophoblast culture to exploreFrontiers in cellular and infection microbiology · 2024Article
- A novel patient-derived meningioma spheroid model as a tool to study and treat epithelial-to-mesenchymal transition (EMT) in meningiomas.Acta neuropathologica communications · 2023Article
- Modeling the human placental barrier to understandFrontiers in cellular and infection microbiology · 2023Review
- Adverse events of recreational cannabis use during pregnancy reported to the French Addictovigilance Network between 2011 and 2020.Scientific reports · 2022Article
- Using high throughput screens to predict miscarriages with placental stem cells and long-term stress effects with embryonic stem cells.Birth defects research · 2022Review
- Article
- Mesenchymal Stem Cells-Induced Trophoblast Invasion Is Reduced in Patients with a Previous History of Preeclampsia.International journal of molecular sciences · 2022Article
- Extravillous trophoblast migration and invasion: Impact of environmental chemicals and pharmaceuticals.Reproductive toxicology (Elmsford, N.Y.) · 2022Review
- The Role of the 3Rs for Understanding and Modeling the Human Placenta.Journal of clinical medicine · 2021Review
- Histological changes in placental rat apoptosis via FasL and cytochromeSaudi journal of biological sciences · 2021Article
- A 3-dimensional microfluidic platform for modeling human extravillous trophoblast invasion and toxicological screening.Lab on a chip · 2021Article
- Establishment of an in vitro placental barrier model cultured under physiologically relevant oxygen levels.Molecular human reproduction · 2020Article
- Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Placental extravillous trophoblast (EVT) invasion is essential in establishing proper blood supply to the fetus during pregnancy. However, traditional 2D in vitro systems do not model the in vivo invasion process in an anatomically-relevant manner. Our objectives were to develop a 3D spheroid model that would allow better emulation of placental invasion in vitro and to characterize the transcriptomic and functional outcomes. HTR8/SVneo EVT cells were self-assembled into 3D spheroids using ultra-low attachment plates. Transcriptomic profiling followed by gene set enrichment and gene ontology analyses revealed major global transcriptomic differences, with significant up-regulations in EVTs cultured as 3D spheroids in canonical pathways and biological processes such as immune response, angiogenesis, response to stimulus, wound healing, and others. These findings were further validated by RT-qPCR, showing significant up-regulations in genes and/or proteins related to epithelial-mesenchymal transition, cell-cell contact, angiogenesis, and invasion/migration. A high-throughput, spheroid invasion assay was applied to reveal the dynamic invasion of EVTs away from the spheroid core into extracellular matrix. Lastly, lipopolysaccharide, dexamethasone, or Δ
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Registered trials
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