ArticleThe Journal of cell biology2019
The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover.
Article in The Journal of cell biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- Dissecting cytoskeletal crosstalk through reconstituted actin-microtubule systems.Molecular biology of the cell · 2026Review
- ROCK inhibition promotes axon and myelin regeneration via PI3K/Akt/GSK3β in a mouse sciatic nerve injury model.International journal of molecular medicine · 2026Article
- The role and regulation of integrins in cell migration and invasion.Nature reviews. Molecular cell biology · 2025Review
- APC orchestrates microtubule dynamics by acting as a positive regulator of KIF2A and a negative regulator of CLASPs.Cell insight · 2025Article
- Focal adhesion-related non-ciliary functions of CEP290.PloS one · 2025Article
- From Crypts to Cancer: A Holistic Perspective on Colorectal Carcinogenesis and Therapeutic Strategies.International journal of molecular sciences · 2024Review
- Focal adhesions are controlled by microtubules through local contractility regulation.The EMBO journal · 2024Article
- Tumor invasiveness is regulated by the concerted function of APC, formins, and Arp2/3 complex.iScience · 2024Article
- Article
- Focal adhesions contain three specialized actin nanoscale layers.Nature communications · 2024Article
- Extracellular matrix sensing via modulation of orientational order of integrins and F-actin in focal adhesions.Life science alliance · 2023Article
- APC-driven actin nucleation powers collective cell dynamics in colorectal cancer cells.iScience · 2023Article
- CLASP2 facilitates dynamic actin filament organization along the microtubule lattice.Molecular biology of the cell · 2023Article
- Confocal Laser Scanning Imaging of Cell Junctions in Human Colon Cancer Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Septins mediate a microtubule-actin crosstalk that enables actin growth on microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The CLIP-170 N-terminal domain binds directly to both F-actin and microtubules in a mutually exclusive manner.The Journal of biological chemistry · 2022Article
- Review
- Subtle changes in crosslinking drive diverse anomalous transport characteristics in actin-microtubule networks.Soft matter · 2021Article
- Article
- New twists in actin-microtubule interactions.Molecular biology of the cell · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Focal adhesion (FA) turnover depends on microtubules and actin. Microtubule ends are captured at FAs, where they induce rapid FA disassembly. However, actin's roles are less clear. Here, we use polarization-resolved microscopy, FRAP, live cell imaging, and a mutant of Adenomatous polyposis coli (APC-m4) defective in actin nucleation to investigate the role of actin assembly in FA turnover. We show that APC-mediated actin assembly is critical for maintaining normal F-actin levels, organization, and dynamics at FAs, along with organization of FA components. In WT cells, microtubules are captured repeatedly at FAs as they mature, but once a FA reaches peak maturity, the next microtubule capture event leads to delivery of an autophagosome, triggering FA disassembly. In APC-m4 cells, microtubule capture frequency and duration are altered, and there are long delays between autophagosome delivery and FA disassembly. Thus, APC-mediated actin assembly is required for normal feedback between microtubules and FAs, and maintaining FAs in a state "primed" for microtubule-induced turnover.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.