Evidence map›Paper›PMID 31470576›Full record

ArticleBiosensors2019

Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate.

Julia Döring, David Rettke, Gerhard Rödel, Tilo Pompe, Kai Ostermann

Open access · goldAbstract read
In one paragraph

Article in Biosensors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Julia DöringInstitute of Genetics, Technische Universität Dresden, Zellescher Weg 20b, 01217 Dresden, Germany. julia.doering1@tu-dresden.de.ORCID 0000-0001-7717-4381
David RettkeInstitute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany.
Gerhard RödelInstitute of Genetics, Technische Universität Dresden, Zellescher Weg 20b, 01217 Dresden, Germany.
Tilo PompeInstitute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany.ORCID 0000-0003-1508-4959
Kai OstermannInstitute of Genetics, Technische Universität Dresden, Zellescher Weg 20b, 01217 Dresden, Germany.
Technische Universität Dresden · DELeipzig University · DE

Funding

Bundesministerium für Bildung und Forschung 13N13798
6 · The paper itself

Abstract

Glyphosate, the most widely used pesticide worldwide, is under debate due to its potentially cancerogenic effects and harmful influence on biodiversity and environment. Therefore, the detection of glyphosate in water, food or environmental probes is of high interest. Currently detection of glyphosate usually requires specialized, costly instruments, is labor intensive and time consuming. Here we present a fast and simple method to detect glyphosate in the nanomolar range based on the surface immobilization of glyphosate's target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) via fusion to the hydrophobin Ccg2 and determination of enzyme activity with a malachite green assay, which is a common photometric technique to measure inorganic phosphate (Pi). The assay demonstrates a new approach for a fast and simple detection of pesticides.

Indexed as

GlycineGlyphosateMembrane Fusion ProteinsGlycineGlyphosateMembrane Fusion ProteinsEPSPSglyphosatehydrophobinimmobilizationmalachite green assay

Identifiers

PMID31470576
PMCPMC6784374
OpenAlexW2970317531

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.