Evidence map›Paper›PMID 31470007›Full record

ArticleGastroenterology2019

Interleukin 1 beta and Matrix Metallopeptidase 3 Contribute to Development of Epidermal Growth Factor Receptor-Dependent Serrated Polyps in Mouse Cecum.

Zhengxiang He, Lili Chen, Grace Chen, Paola Smaldini, Gerold Bongers, Jovani Catalan-Dibene, Glaucia C Furtado, Sergio A Lira

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gastroenterology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
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  11. Fibroblast Reprogramming in Gastrointestinal Cancer.Frontiers in cell and developmental biology · 2020
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Zhengxiang HePrecision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York.
Lili ChenPrecision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York.
Grace ChenPrecision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York.
Paola SmaldiniPrecision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York.
Gerold BongersDepartment of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York.
Jovani Catalan-DibenePrecision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York.
Glaucia C FurtadoPrecision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York.
Sergio A LiraPrecision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York. Electronic address: sergio.lira@mssm.edu.
Icahn School of Medicine at Mount Sinai · US

Funding

Mechanisms associated with flares and remission in colitisR01DK110352 · NIDDK · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI LIRA, SERGIO A. · 2017 to 2025
$5.1M
Molecular pathogenesis of intestinal serrated polypsR01CA161373 · NCI · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI LIRA, SERGIO A. · 2011 to 2020
$3.7M
NCI NIH HHS R01 CA161373NIDDK NIH HHS R01 DK110352
6 · The paper itself

Abstract

BACKGROUND &

aimsTransgenic mice (HBUS) that express the epidermal growth factor receptor (EGFR) ligand HBEGF (heparin-binding epidermal growth factor-like growth factor) and a constitutively active G protein-coupled receptor (US28) in intestinal epithelial cells develop serrated polyps in the cecum. Development of serrated polyps depends on the composition of the gut microbiota and is associated with bacterial invasion of the lamina propria, accompanied by induction of inflammation and up-regulation of interleukin 1 beta (IL1B) and matrix metalloproteinase (MMP) 3 in the cecum. We investigated the mechanisms by which these changes contribute to development of serrated polyps.

methodsWe performed studies with C57BL/6 (control) and HBUS mice. To accelerate polyp development, we increased the exposure of the bacteria to the lamina propria by injecting HBUS mice with diphtheria toxin, which binds transgenic HBEGF expressed by the epithelial cells and causes apoptosis. Mice were given injections of IL1B-neutralizing antibody and the MMP inhibitor N-isobutyl-N-(4-methoxyphenylsulfonyl)glycyl hydroxamic acid. Intestinal tissues were collected from mice and analyzed by histology, reverse-transcription polymerase chain reaction, enzyme-linked immunosorbent assay, immunofluorescence, and flow cytometry. We examined fibroblast subsets in polyps using single-cell RNA sequencing.

resultsAdministration of diphtheria toxin to HBUS mice accelerated development of serrated polyps (95% of treated mice developed polyps before 100 days of age, compared with 53% given vehicle). IL1B stimulated subsets of platelet-derived growth factor receptor alpha

conclusionsIn studies of mice, we found that barrier breakdown and expression of inflammatory factors contribute to development of serrated polyps. Subsets of cecal PDGFRA

Indexed as

AnimalsApoptosisCecumColonic PolypsDiphtheria ToxinDisease Models, AnimalEpithelial CellsErbB ReceptorsFibroblastsGefitinibHeparin-binding EGF-like Growth FactorHumansHydroxamic AcidsInterleukin-1betaIntestinal MucosaMatrix Metalloproteinase 3Diphtheria ToxinEGFR protein, mouseErbB ReceptorsGefitinibHbegf protein, mouseHeparin-binding EGF-like Growth FactorHydroxamic AcidsIL1B protein, mouseInterleukin-1betaMatrix Metalloproteinase 3Matrix Metalloproteinase InhibitorsMmp3 protein, mouseN-isobutyl-N-(4-methoxyphenylsulfonyl)glycylhydroxamic acidReceptor, Platelet-Derived Growth Factor alphaSulfonamidesBarrier BreakdownCytokineNeoplasmStroma

Identifiers

PMID31470007
PMCPMC7006742
OpenAlexW2970518609

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.