ArticleInvestigative ophthalmology & visual science2019
Physiologic Electrical Fields Direct Retinal Ganglion Cell Axon Growth In Vitro.
Article in Investigative ophthalmology & visual science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
- Population-scale analysis of frequency-dependent calcium dynamics in retinal ganglion cells under electric field stimulation.Scientific reports · 2026Article
- Population-Scale Analysis of Frequency-Dependent Calcium Dynamics in Retinal Ganglion Cells Under Electric Field Stimulation.bioRxiv : the preprint server for biology · 2026Article
- A Microscale-Optical Interface to Examine Electric Field-Induced Cell Motility Within Whole-Eye Facsimiles.Micro · 2025Article
- Electric field stimulation directs target-specific axon regeneration and partial restoration of vision after optic nerve crush injury.PloS one · 2025Article
- Transcriptomic Analysis Reveals the Heterogeneous Role of Conducting Films Upon Electrical Stimulation.Advanced healthcare materials · 2024Article
- Noninvasive Light Flicker Stimulation Promotes Optic Nerve Regeneration by Activating Microglia and Enhancing Neural Plasticity in Zebrafish.Investigative ophthalmology & visual science · 2024Article
- Extrinsic electric field modulates neuronal development and increases photoreceptor population in retinal organoids.Frontiers in neuroscience · 2024Article
- Unlocking the potential for optic nerve regeneration over long distances: a multi-therapeutic intervention.Frontiers in neurology · 2024Review
- Asymmetric charge balanced waveforms direct retinal ganglion cell axon growth.Scientific reports · 2023Article
- Integrin-mediated electric axon guidance underlying optic nerve formation in the embryonic chick retina.Communications biology · 2023Article
- An organ-on-chip device with integrated charge sensors and recording microelectrodes.Scientific reports · 2023Article
- Analysis of Electric Field Stimulation in Blue Light Stressed 661W Cells.International journal of molecular sciences · 2023Article
- The Influence of Electrode Properties on Induced Voltage Gradient Along the Rat Optic Nerve.IEEE journal of electromagnetics, RF and microwaves in medicine and biology · 2022Article
- A Subdural Bioelectronic Implant to Record Electrical Activity from the Spinal Cord in Freely Moving Rats.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Localized electrical stimulation triggers cell-type-specific proliferation in biofilms.Cell systems · 2022Article
- Direct Current Electric Field Coordinates the Migration of BV2 Microglia via ERK/GSK3β/Cofilin Signaling Pathway.Molecular neurobiology · 2022Article
- Short-term bioelectric stimulation of collective cell migration in tissues reprograms long-term supracellular dynamics.PNAS nexus · 2022Article
- Mathematical models of neuronal growth.Biomechanics and modeling in mechanobiology · 2022Review
- Non-Arteritic Anterior Ischemic Optic Neuropathy: Challenges for the Future.Frontiers in ophthalmology · 2022Article
- Optic chiasmatic potential by endoscopically implanted skull base microinvasive biosensor: a brain-machine interface approach for anterior visual pathway assessment.Theranostics · 2022Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Purpose: The purpose of this study was to characterize the ability of applied electrical fields (EFs) to direct retinal ganglion cell (RGC) axon growth as well as to assess whether Rho GTPases play a role in translating electrical cues to directional cues. Methods: Full-thickness, early postnatal mouse retina was cultured in electrotaxis chambers and exposed to EFs of varying strengths (50-200 mV/mm). The direction of RGC axon growth was quantified from time-lapsed videos. The rate of axon growth and responsiveness to changes in EF polarity were also assessed. The effect of toxin B, a broad-spectrum inhibitor of Rho GTPase signaling, and Z62954982, a selective inhibitor of Rac1, on EF-directed growth was determined. Results: In the absence of an EF, RGC axons demonstrated indiscriminate directional growth from the explant edge. Retinal cultures exposed to an EF of 100 and 200 mV/mm showed markedly asymmetric growth, with 74.2% and 81.2% of axons oriented toward the cathode, respectively (P < 0.001). RGC axons responded to acute changes in EF polarity by redirecting their growth toward the "new" cathode. This galvanotropic effect was partially neutralized by toxin B and Rac1 inhibitor Z62954982. Conclusions: RGC axons exhibit cathode-directed growth in the presence of an EF. This effect is mediated in part by the Rho GTPase signaling cascade.
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