Evidence map›Paper›PMID 31468594›Full record

ArticleCell proliferation2019

LncRNA LOXL1-AS1 facilitates the tumorigenesis and stemness of gastric carcinoma via regulation of miR-708-5p/USF1 pathway.

Qi Sun, Jian Li, Fan Li, Huanqin Li, Songhua Bei, Xiaohong Zhang, Li Feng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed, 1 pooled it
4.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 1 synthesis or guideline pooled it, 104 citations in OpenAlex.

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  15. [LncRNA LINC00342 regulates gastric cancer cell proliferation, migration and invasion by targeting miR-596].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Qi SunEndoscopy Center, Minhang Hospital, Fudan University, Shanghai, China.ORCID https://orcid.org/0000-0002-5679-5346
Jian LiEndoscopy Center, Minhang Hospital, Fudan University, Shanghai, China.
Fan LiEndoscopy Center, Minhang Hospital, Fudan University, Shanghai, China.
Huanqin LiEndoscopy Center, Minhang Hospital, Fudan University, Shanghai, China.
Songhua BeiEndoscopy Center, Minhang Hospital, Fudan University, Shanghai, China.
Xiaohong ZhangEndoscopy Center, Minhang Hospital, Fudan University, Shanghai, China.
Li FengEndoscopy Center, Minhang Hospital, Fudan University, Shanghai, China.
Fudan University · CN

Funding

Minhang District Subject Pilot Talent ProjectMinhang District Talent Development Special Fund ProjectMinhang District University Building Project 2017MWDXK03
6 · The paper itself

Abstract

objectivesAs one of the most life-threatening malignancies, gastric cancer is the third contributor of cancer mortalities globally. Increasing studies have proven the regulatory roles of lncRNAs in the development of diverse malignant tumours. But little is known about its function and molecular mechanism in gastric carcinoma. MATERIALS AND

methodsRT-qPCR was performed to measure the expression pattern of LOXL1-AS1 in gastric cancer. To ascertain its definite role, CCK-8, EdU, Western blot, transwell and sphere formation assays were adopted. RNA pull-down, RIP, ChIP and luciferase reporter assays were carried out to investigate the molecular mechanism of LOXL1-AS1 in gastric carcinoma.

resultsLOXL1-AS1 was highly expressed in tissues and cells of gastric cancer. The upregulation of LOXL1-AS1 predicted poor prognosis in gastric carcinoma. Our findings demonstrated that LOXL1-AS1 accelerated the deterioration of gastric cancer by inducing cell proliferation, migration, EMT and stemness. Moreover, the expression of USF1 in gastric cancer was higher than in normal control and LOXL1-AS1 negatively modulated USF1. Functionally, LOXL1-AS1 acted as a ceRNA to upregulate USF1 via sponging miR-708-5p. Besides, we confirmed USF1 promoted the transcription of stemness marker SOX2. Rescue experiments testified the stimulative role of LOXL1-AS1/miR-708-5p/USF1 pathway in gastric cancer progression. It was also validated that LOXL1-AS1 facilitated cell growth of gastric carcinoma in vivo.

conclusionsOur study unravelled that LOXL1-AS1/miR-708-5p/USF1 pathway contributed to the development of gastric cancer.

Indexed as

Amino Acid OxidoreductasesCarcinogenesisCell Line, TumorCell ProliferationCell Transformation, NeoplasticGene Expression Regulation, NeoplasticHumansMicroRNAsRNA, Long NoncodingStomach NeoplasmsUpstream Stimulatory FactorsAmino Acid OxidoreductasesLOXL1 protein, humanMicroRNAsMIRN708 microRNA, humanRNA, Long NoncodingUpstream Stimulatory FactorsUSF1 protein, humanCSCgastric cancerLOXL1-AS1miR-708-5pUSF1

Identifiers

PMID31468594
PMCPMC6869681
OpenAlexW2971309438

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.