ArticleJournal of anatomy2020
In vivo identification of arteries and veins using two-photon excitation elastin autofluorescence.
Article in Journal of anatomy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Volumetric photoacoustic imaging of elastin and age-related remodeling using near-infrared probe ElaNIR.Photoacoustics · 2026Article
- Metabolism and oxygen tension in Alzheimer's disease mouse model using two-photon FLIM-PLIM microscopy.Neurophotonics · 2026Article
- Rapid autofluorescence based 3D optical imaging of the pancreatic cancer milieu at mesoscopic scale - stain-free volumetric segmentation.Scientific reports · 2026Article
- A new method for visualizing cerebrovascular vessels.Frontiers in molecular neuroscience · 2026Article
- Expression of Elastin, F-Box and WD-40 Domain-Containing Protein 2, Fibrillin-1, and Alpha-Smooth Muscle Actin in Utilized Blood Vessels for explant culture-A New 3D in Vitro Vascular Model from Bovine Legs.Cell biochemistry and biophysics · 2025Article
- Arteries and veins in awake mice using two-photon microscopy.Journal of anatomy · 2025Article
- Calcification of the elastic component: the impact on the cardiovascular system.Frontiers in cardiovascular medicine · 2025Review
- Phasor-FLIM and SHG imaging for quantitative analysis of lung cancer autofluorescence.Computational and structural biotechnology journal · 2025Article
- To label or not: the need for validation in label-free imaging.Journal of biomedical optics · 2024Review
- The optimization and application of photodynamic diagnosis and autofluorescence imaging in tumor diagnosis and guided surgery: current status and future prospects.Frontiers in oncology · 2024Review
- Non-invasive assessment of hair regeneration in androgenetic alopecia miceBiomedical optics express · 2023Article
- A Pleomorphic Puzzle: Heterogeneous Pulmonary Vascular Occlusions in Patients with COVID-19.International journal of molecular sciences · 2022Article
- Multimodal microscopy for the simultaneous visualization of five different imaging modalities using a single light source.Biomedical optics express · 2021Article
- Widely-tunable synchronisation-free picosecond laser source for multimodal CARS, SHG, and two-photon microscopy.Biomedical optics express · 2021Article
- Super resolution measurement of collagen fibers in biological samples: Validation of a commercial solution for multiphoton microscopy.PloS one · 2020Article
- Histological findings for the absorption of small and large liposomes - the basis of future drug delivery and contrast media systems.Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologieArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Distinguishing arteries from veins in vivo has a great significance in clinical practices and preclinical studies. Optical imaging methods such as two-photon microscopy can provide high-resolution morphological information of tissue and are therefore extremely suitable for imaging small blood vessels. However, few optical imaging methods allow in vivo identification of arteries and veins merely utilizing the autofluorescence signal of blood vessels. In this report, we found the arterial wall generates a remarkably stronger two-photon excitation autofluorescence (TPEA) signal compared with the venous wall based on BALB/c mice. According to histological analysis and fluorescence characteristic measurement, the contrast signal is confirmed to be from elastin fibers. Employing this unique feature, we propose an objective and effective artery-vein separation strategy that considers the presence of the elastin-TPEA border as the indicator of arteries. Using this strategy, the arterial and venous networks of the dorsal skin and cerebral cortex of BALB/c mice are demonstrated to be excellently mapped and accurately separated in vivo without depending on any exogenous contrast agent, empirical knowledge, and algorithm. This study may provide a novel technique for mapping arterial and venous networks for anatomic research as well as an extra aid to basic researches on the mechanism, diagnosis, and treatment of blood vessel-related diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.