ArticlePlant direct2019
An optimized protocol for isolation of high-quality RNA through laser capture microdissection of leaf material.
Article in Plant direct, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 16 citations in OpenAlex.
- Improved laser capture microdissection combined with transcriptome sequencing reveals gene expression reprogramming during rice panicle branch meristems differentiation.BMC plant biology · 2026Article
- A transcription factor ensemble orchestrates bundle sheath expression in rice.Nature communications · 2025Article
- Xylan-based nanocompartments orchestrate plant vessel wall patterning.Nature plants · 2022Article
- Engineering chloroplast development in rice through cell-specific control of endogenous genetic circuits.Plant biotechnology journal · 2021Article
- Photosynthesis-independent production of reactive oxygen species in the rice bundle sheath during high light is mediated by NADPH oxidase.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants.3 Biotech · 2020Article
- An optimized protocol for isolation of high-quality RNA through laser capture microdissection of leaf material.Plant direct · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laser Capture Microdissection is a powerful tool that allows thin slices of specific cell types to be separated from one another. However, the most commonly used protocol, which involves embedding tissue in paraffin wax, results in severely degraded RNA. Yields from low abundance cell types of leaves are particularly compromised. We reasoned that the relatively high temperature used for sample embedding, and aqueous conditions associated with sample preparation prior to microdissection contribute to RNA degradation. Here, we describe an optimized procedure to limit RNA degradation that is based on the use of low-melting-point wax as well as modifications to sample preparation prior to dissection, and isolation of paradermal, rather than transverse sections. Using this approach, high-quality RNA suitable for down-stream applications such as quantitative reverse transcriptase-polymerase chain reactions or RNA-sequencing is recovered from microdissected bundle sheath strands and mesophyll cells of leaf tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.