ArticleScientific reports2019
Environmental DNA size sorting and degradation experiment indicates the state of Daphnia magna mitochondrial and nuclear eDNA is subcellular.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic review and meta-analysis: Water type and temperature affect environmental DNA decay.Molecular ecology resources · 2022Pooled it
- Comparative Decay Dynamics and Detectability of eDNA and eRNA in Connected and Isolated Freshwater Mesocosms Using Digital PCR.Molecular ecology resources · 2025Article
- Polymer Length Governs DNA Adsorption Dynamics on Mineral Surfaces.Environmental science & technology · 2025Article
- Differential decay of multiple environmental nucleic acid components.Scientific reports · 2025Article
- First national survey of terrestrial biodiversity using airborne eDNA.Scientific reports · 2025Article
- Detection, movement and persistence of invertebrate eDNA in groundwater.Scientific reports · 2024Article
- Larger particle size distribution of environmental RNA compared to environmental DNA: a case study targeting the mitochondrial cytochrome b gene in zebrafish (Danio rerio) using experimental aquariums.Die Naturwissenschaften · 2024Article
- Article
- Utilizing the state of environmental DNA (eDNA) to incorporate time-scale information into eDNA analysis.Proceedings. Biological sciences · 2023Review
- Signal and noise in metabarcoding data.PloS one · 2023Article
- Interrogating 1000 insect genomes for NUMTs: A risk assessment for estimates of species richness.PloS one · 2023Article
- Article
- Development of an eDNA metabarcoding tool for surveying the world's largest amphibian.Current zoology · 2022Article
- Why eDNA fractions need consideration in biomonitoring.Molecular ecology resources · 2022Review
- Environmental DNA provides higher resolution assessment of riverine biodiversity and ecosystem function via spatio-temporal nestedness and turnover partitioning.Communications biology · 2021Article
- A novel metabarcoding primer pair for environmental DNA analysis of Cephalopoda (Mollusca) targeting the nuclear 18S rRNA region.Royal Society open science · 2021Article
- Environmental (e)RNA advances the reliability of eDNA by predicting its age.Scientific reports · 2021Article
- Water pre-filtration methods to improve environmental DNA detection by real-time PCR and metabarcoding.PloS one · 2021Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental DNA analysis has emerged as a key component of biodiversity and environmental monitoring. However, the state and fate of eDNA in natural environments is still poorly understood for many ecological systems. Here we assess the state and fate of eDNA derived from the water flea, Daphnia magna, using a full factorial mesocosm experiment. We measured the quantity and degradation of eDNA over a two month period across a range of filters differing in pore size (0, 0.2, 1 and 10 µm), which spans the range of eDNA source material including subcellular, cellular and tissue. We also used two primer sets targeting mitochondrial (COI) and nuclear (18S) genomic regions. Our findings demonstrated that eDNA was most prevalent in the effluent water, but also reliably detected on the 0.2 μm filter, suggesting subcellular material is the predominate state of eDNA. Temporal eDNA quantity dynamics followed an exponential decay function over the course of 6-17 days, demonstrating a predictable decline in eDNA concentration. Nuclear eDNA was more abundant than mitochondrial eDNA, which may be a result of greater primer affinity, or indicate greater availability of nuclear eDNA gene targets in the environment. In contrast to two previous size-sorting experiments, which utilizing fish eDNA, our findings suggest that the state of invertebrate eDNA is much smaller than previously suspected. Overall, our data suggest that the detection of eDNA greatly depends on our knowledge of the state and fate of eDNA, which differ among species, and likely across environmental conditions.
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