ArticleScientific reports2019
Biosynthesis of Hyaluronic acid polymer: Dissecting the role of sub structural elements of hyaluronan synthase.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- Tailored molecular weight hyaluronic acid production by engineered Lactococcus lactis.Microbial cell factories · 2026Article
- Targeting CD44-Hyaluronic Acid Signalling in Obesity Treatment: Insights from Small Molecules and Nanobioconjugates.Nutrition and metabolic insights · 2026Review
- Molecular weight regulation strategies in microbial synthesis of hyaluronic acid: current status and future perspectives.World journal of microbiology & biotechnology · 2025Review
- Novel Function for Endothelial Protease-Activated Receptors in Modulating Insulin Receptor Activity With Implications for Diabetes.Arteriosclerosis, thrombosis, and vascular biology · 2025Article
- Hyaluronic Acid and Its Synthases-Current Knowledge.International journal of molecular sciences · 2025Review
- Drug-free hyaluronic acid-microneedle with unexpected inhibition activity on benzalkonium chloride-induced corneal inflammation and stromal scarring.Materials today. Bio · 2025Article
- CRISPRi-assisted metabolic engineering of cyanobacteria for photosynthetic hyaluronic acid from COJournal of biological engineering · 2025Article
- Regulating cellular metabolism and morphology to achieve high-yield synthesis of hyaluronan with controllable molecular weights.Nature communications · 2025Article
- Combined Hyaluronic Acid Nanobioconjugates Impair CD44-Signaling for Effective Treatment Against Obesity: A Review of Comparison with Other Actors.International journal of nanomedicine · 2025Review
- Enzyme variants in biosynthesis and biological assessment of different molecular weight hyaluronan.AMB Express · 2024Article
- Review
- Bioinformatics characterization of BcsA-like orphan proteins suggest they form a novel family of pseudomonad cyclic-β-glucan synthases.PloS one · 2023Article
- Hyaluronic acid production and characterization by novel Bacillus subtilis harboring truncated Hyaluronan Synthase.AMB Express · 2022Article
- Prospective bacterial and fungal sources of hyaluronic acid: A review.Computational and structural biotechnology journal · 2022Review
- Shock-Induced Damage Mechanism of Perineuronal Nets.Biomolecules · 2021Article
- Collagen- and hyaluronic acid-based hydrogels and their biomedical applications.Materials science & engineering. R, Reports : a review journal · 2021Article
- Inhibition of hyaluronan secretion by novel coumarin compounds and chitin synthesis inhibitors.Glycobiology · 2021Article
- Indirect Pathway Metabolic Engineering Strategies for Enhanced Biosynthesis of Hyaluronic Acid in EngineeredFrontiers in bioengineering and biotechnology · 2021Article
- The Role of Glycocalyx and Caveolae in Vascular Homeostasis and Diseases.Frontiers in physiology · 2020Review
- Hyaluronic acid in Dentoalveolar regeneration: Biological rationale and clinical applications.Journal of oral biology and craniofacial researchArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hyaluronic acid (HA) based biomaterials have several biomedical applications. HA biosynthesis is catalysed by hyaluronan synthase (HAS). The unavailability of 3-D structure of HAS and gaps in molecular understanding of HA biosynthesis process pose challenges in rational engineering of HAS to control HA molecular weight and titer. Using in-silico approaches integrated with mutation studies, we define a dictionary of sub-structural elements (SSE) of the Class I Streptococcal HAS (SeHAS) to guide rational engineering. Our study identifies 9 SSE in HAS and elucidates their role in substrate and polymer binding and polymer biosynthesis. Molecular modelling and docking assessment indicate a single binding site for two UDP-substrates implying conformationally-driven alternating substrate specificities for this class of enzymes. This is the first report hypothesizing the involvement of sites from SSE5 in polymer binding. Mutation at these sites influence HA production, indicating a tight coupling of polymer binding and synthase functions. Mutation studies show dispensable role of Lys-139 in substrate binding and a key role of Gln-248 and Thr-283 in HA biosynthesis. Based on the functional architecture in SeHAS, we propose a plausible three-step polymer extension model from its reducing end. Together, these results open new avenues for rational engineering of Class I HAS to study and regulate its functional properties and enhanced understanding of glycosyltransferases and processive enzymes.
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