Evidence map›Paper›PMID 31463640›Full record

ArticleMolecular biology reports2019

Transcript profiling of chickpea pod wall revealed the expression of floral homeotic gene AGAMOUS-like X2 (CaAGLX2).

Jagadale Mahesh Vasantrao, Indrani K Baruah, Debashis Panda, Mamta Bhattacharjee, Sumita Acharjee, Bidyut K Sarmah

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Article in Molecular biology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Jagadale Mahesh VasantraoDepartment of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India.
Indrani K BaruahDepartment of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India.
Debashis PandaDistributed Information Centre, Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India.
Mamta BhattacharjeeDepartment of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India.
Sumita AcharjeeDepartment of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India. sumita.aus@gmail.com.
Bidyut K SarmahDepartment of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India. bidyutsarmah@aau.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The differentially expressed genes in the chickpea pod wall have been identified for the first time using a forward suppression subtractive hybridization (SSH) library. In all, 226 clones of SSH library were sequenced and analyzed. A total of 179 high-quality expressed sequence tags (ESTs) were generated and based on the CAP3 assembly of these ESTs, 126 genes (97 singletons and 29 contigs) were computationally annotated. The mapping of 88.26% ESTs by gene ontology (GO) annotation distributed them into 751 GO terms of three categories, cellular location, molecular function, and biological process. The KEGG pathway analysis revealed 45 ESTs are involved in 49 different biological pathways. Also, 67 ESTs encodes four different classes of enzymes such as oxidoreductases (29), transferase (20), hydrolases (16) and isomerase (2). Six genes were selected and subjected to qPCR analysis, of these, two genes (FHG Floral homeotic AGAMOUS-like isoform X2, MADS1 MADS-box transcription factor) showed significant up-regulation in the pod wall compared to leaves. Surprisingly, one of the MADS1 box gene, FHG (CaAGLX2), responsible for flower development expressed in the pod wall. Therefore, understanding its specific role in the pod wall could be interesting. Thus, the transcript dynamics of the chickpea pod wall revealed differentially expressed genes in the pod wall, which may be participating in the metabolic build-up of both pod wall and seeds.

Indexed as

CicerComputational BiologyFlowersGene Expression Regulation, PlantMADS Domain ProteinsPlant ProteinsSeedsSubtractive Hybridization TechniquesTranscriptomeMADS Domain ProteinsPlant ProteinsChickpea pod wallFloral homeotic AGAMOUS-like isoform X2Gene expressionMADS-box transcription factorSuppression subtractive hybridization

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.