ArticlePlant methods2019
A highly mutagenised barley (
Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Functional analysis of HvSNAC1 in stomatal dynamics and drought adaptation.Journal of applied genetics · 2025Article
- Partial redundancy buffers deleterious effects of mutating DNA methyltransferase 1-1 (MET1-1) in polyploid wheat.Journal of experimental botany · 2025Article
- Boosting photosynthesis opens new opportunities for agriculture sustainability and circular economy: The BEST-CROP research and innovation action.The Plant journal : for cell and molecular biology · 2025Review
- Development and Characterization of a New TILLING Population for Forward and Reverse Genetics in Barley (Plants (Basel, Switzerland) · 2024Article
- Genetic Screening of Factors in the Plant Protein Secretion.Methods in molecular biology (Clifton, N.J.) · 2024Article
- HvSL1 and HvMADS16 promote stamen identity to restrict multiple ovary formation in barley.Journal of experimental botany · 2023Article
- Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment.aBIOTECH · 2023Article
- Epigenetic Changes Occurring in Plant Inbreeding.International journal of molecular sciences · 2023Review
- Drought and recovery in barley: key gene networks and retrotransposon response.Frontiers in plant science · 2023Article
- Augmenting tomato functional genomics with a genome-wide induced genetic variation resource.Frontiers in plant science · 2023Article
- Is it the end of TILLING era in plant science?Frontiers in plant science · 2023Review
- FIND-IT: Accelerated trait development for a green evolution.Science advances · 2022Article
- A reference-guided TILLING by amplicon-sequencing platform supports forward and reverse genetics in barley.Plant communications · 2022Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Cloning the barley nec3 disease lesion mimic mutant using complementation by sequencing.The plant genome · 2022Article
- Measuring the frequency and distribution of meiotic crossovers in homozygous barley inbred lines.Frontiers in plant science · 2022Article
- Optimized methods for random and targeted mutagenesis in field pea (Frontiers in plant science · 2022Article
- An Induced Mutation inFrontiers in plant science · 2021Article
- The complete chloroplast genome sequence ofMitochondrial DNA. Part B, Resources · 2021Article
- Downregulation of BarleyFrontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe developed and characterised a highly mutagenised TILLING population of the barley (
resultsSequencing 20 plants from the population using whole exome capture revealed that the mutation density in this population is high (one mutation every 154 kb), and consequently even in this small number of plants we identified several interesting mutations. We also recorded some issues with seed availability and germination. We subsequently designed and applied a simple two-dimensional pooling strategy to identify mutations in varying numbers of specific target genes by Illumina short read pooled-amplicon sequencing and subsequent deconvolution. In parallel we assembled a collection of semi-sterile mutants from the population and used a custom exome capture array targeting the 46 candidate meiotic genes to identify potentially causal mutations.
conclusionsWe developed a highly mutagenised barley TILLING population in the transformation competent cultivar Golden Promise. We used novel and cost-efficient screening approaches to successfully identify a broad range of potentially deleterious variants that were subsequently validated by Sanger sequencing. These resources combined with a high-quality genome reference sequence opens new possibilities for efficient functional gene validation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.