ArticleScientific reports2019
Complex Susceptibilities and Chiroptical Effects of Collagen Measured with Polarimetric Second-Harmonic Generation Microscopy.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 49 citations in OpenAlex.
- Double Stokes polarimetry of birefringent structures.Biomedical optics express · 2026Article
- Fibre orientations in collagen-containing tissues revealed with computational scattered light imaging and polarimetric second harmonic generation microscopy.Scientific reports · 2025Article
- Polar organization of H&E dyes in histology tissue revealed by polarimetric nonlinear microscopy.Biomedical optics express · 2025Article
- Double Stokes polarimetric microscopy for chiral fibrillar aggregates.Scientific reports · 2025Article
- Exploring nonlinear optical properties in a hybrid dihydrogen phosphate system: an experimental and theoretical approach.Journal of molecular modeling · 2024Article
- Effect of out of plane orientation on polarization second harmonic generation of single collagen fibrils.Biomedical optics express · 2023Article
- Polarimetric second-harmonic generation microscopy of partially oriented fibers I: Digital modeling.Biophysical journal · 2023Article
- Polarimetric second harmonic generation microscopy of partially oriented fibers II: Imaging study.Biophysical journal · 2023Article
- High numerical aperture imaging allows chirality measurement in individual collagen fibrils using polarization second harmonic generation microscopy.Nanophotonics (Berlin, Germany) · 2023Article
- Unsupervised determination of lung tumor margin with widefield polarimetric second-harmonic generation microscopy.Scientific reports · 2022Article
- Machine learning-enabled cancer diagnostics with widefield polarimetric second-harmonic generation microscopy.Scientific reports · 2022Article
- Examining lysyl oxidase-like modulation of collagen architecture in 3D spheroid models of idiopathic pulmonary fibrosis via second-harmonic generation microscopy.Journal of biomedical optics · 2021Article
- Dual-LC PSHG microscopy for imaging collagen type I and type II gels with pixel-resolution analysis.Biomedical optics express · 2021Article
- Three-dimensional characterization of collagen remodeling in cell-seeded collagen scaffolds via polarization second harmonic generation.Biomedical optics express · 2021Article
- Characterization of thymine microcrystals by CARS and SHG microscopy.Scientific reports · 2020Article
- Characterization of pathological thyroid tissue using polarization-sensitive second harmonic generation microscopy.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- Polarimetric second-harmonic generation microscopy of the hierarchical structure of collagen in stage I-III non-small cell lung carcinoma.Biomedical optics express · 2020Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Nonlinear optical properties of collagen type-I are investigated in thin tissue sections of pig tendon as a research model using a complete polarimetric second-harmonic generation (P-SHG) microscopy technique called double Stokes-Mueller polarimetry (DSMP). Three complex-valued molecular susceptibility tensor component ratios are extracted. A significant retardance is observed between the chiral susceptibility component and the achiral components, while the achiral components appear to be in phase with each other. The DSMP formalism and microscopy measurements are further used to explain and experimentally validate the conditions required for SHG circular dichroism (SHG-CD) of collagen to occur. The SHG-CD can be observed with the microscope when: (i) the chiral second-order susceptibility tensor component has a non-zero value, (ii) a phase retardance is present between the chiral and achiral components of the second-order susceptibility tensor and (iii) the collagen fibres are tilted out of the image plane. Both positive and negative areas of SHG-CD are observed in microscopy images, which relates to the anti-parallel arrangement of collagen fibres in different fascicles of the tendon. The theoretical formalism and experimental validation of DSMP imaging technique opens new opportunities for ultrastructural characterisation of chiral molecules, in particular collagen, and provides basis for the interpretation of SHG-CD signals. The nonlinear imaging of chiroptical parameters offers new possibilities to further improve the diagnostic sensitivity and/or specificity of nonlinear label-free histopathology.
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