ArticleScientific reports2019
A label-free optical whole-cell Escherichia coli biosensor for the detection of pyrethroid insecticide exposure.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Research Progress of Terahertz Technology in Microbiology.Biosensors · 2026Review
- Bacteria as living biosensors for DNA.Nature reviews bioengineering · 2026Article
- A Review on the Application of Biosensors for Monitoring Emerging Contaminants in the Water Environment.Sensors (Basel, Switzerland) · 2025Review
- Sustainable Integration of Nanobiosensors in Biomedical and Civil Engineering: A Comprehensive Review.ACS omega · 2025Review
- Next-Gen Nano Biosensor Technologies to Monitor Carbapenem Resistance for Personalized Medicine.Indian journal of microbiology · 2025Article
- A review of biosensor for environmental monitoring: principle, application, and corresponding achievement of sustainable development goals.Bioengineered · 2023Review
- An Adenosylcobalamin Specific Whole-Cell Biosensor.Advanced healthcare materials · 2023Article
- Automated design of protein-binding riboswitches for sensing human biomarkers in a cell-free expression system.Nature communications · 2023Article
- Microbial Cells as a Microrobots: From Drug Delivery to Advanced Biosensors.Biomimetics (Basel, Switzerland) · 2023Review
- Reprogramming Synthetic Cells for Targeted Cancer Therapy.ACS synthetic biology · 2022Article
- Article
- Rapid-format recombinant antibody-based methods for the diagnosis ofFrontiers in microbiology · 2022Review
- Microbial whole-cell biosensors: Current applications, challenges, and future perspectives.Biosensors & bioelectronics · 2021Review
- The Potential Use of a Thin Film Gold Electrode Modified with Laccases for the Electrochemical Detection of Pyrethroid Metabolite 3-Phenoxybenzaldehyde.Materials (Basel, Switzerland) · 2021Article
- A Review on Biosensors and Recent Development of Nanostructured Materials-Enabled Biosensors.Sensors (Basel, Switzerland) · 2021Review
- Emerging biosensors in detection of natural products.Synthetic and systems biotechnology · 2020Review
- Biosensors for the detection of respiratory viruses: A review.Talanta open · 2020Review
- Review
- Comparative analysis on bioactivity against three stored insects of Ligusticum pteridophyllum Franch. rhizomes essential oil and supercritical fluid (SFE-COEnvironmental science and pollution research international · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
There is a growing need for low-cost, portable technologies for the detection of threats to the environment and human health. Here we propose a label-free, optical whole-cell Escherichia coli biosensor for the detection of 3-phenoxybenzoic acid (3-PBA), a biomarker for monitoring human exposure to synthetic pyrethroid insecticides. The biosensor functions like a competitive ELISA but uses whole-cells surface displaying an anti-3-PBA VHH as the detection element. When the engineered cells are mixed with 3-PBA-protein conjugate crosslinking that can be visually detected occurs. Free 3-PBA in samples competes with these crosslinks, leading to a detectable change in the output. The assay performance was improved by coloring the cells via expression of the purple-blue amilCP chromoprotein and the VHH expression level was reduced to obtain a limit of detection of 3 ng/mL. The optimized biosensor exhibited robust function in complex sample backgrounds such as synthetic urine and plasma. Furthermore, lyophilization enabled storage of biosensor cells for at least 90 days without loss of functionality. Our whole-cell biosensor is simple and low-cost and therefore has potential to be further developed as a screening tool for monitoring exposure to pyrethroids in low-resource environments.
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Registered trials
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