Evidence map›Paper›PMID 31462299›Full record

ArticleStem cell research & therapy2019

Bone marrow concentrate-induced mesenchymal stem cell conditioned medium facilitates wound healing and prevents hypertrophic scar formation in a rabbit ear model.

Ching-Hsuan Hu, Yi-Wen Tseng, Chih-Yung Chiou, Kuan-Chun Lan, Chih-Hung Chou, Chun-San Tai, Hsien-Da Huang, Chiung-Wen Hu, Ko-Hsun Liao, Shiow-Shuh Chuang and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
5.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Ching-Hsuan HuDepartment of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Chang Gung Medical College and Chang Gung University, Taoyuan, Taiwan.
Yi-Wen TsengDepartment of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Chang Gung Medical College and Chang Gung University, Taoyuan, Taiwan.
Chih-Yung ChiouInstitute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
Kuan-Chun LanInstitute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, 300, Taiwan.
Chih-Hung ChouDepartment of Biological Science and Technology, Center for Intelligent Drug Systems and Smart Bio-devices (IDS²B), National Chiao Tung University, Hsinchu, 300, Taiwan.
Chun-San TaiDepartment of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan.
Hsien-Da HuangWarshel Institute for Computational Biology, School of Life and Health Sciences, School of Sciences and Engineering, The Chinese University of Hong Kong, Shenzhen, 518172, China.
Chiung-Wen HuDepartment of Public Health, Chung Shan Medical University, Taichung, 402, Taiwan.
Ko-Hsun LiaoInstitute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
Shiow-Shuh ChuangDepartment of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Chang Gung Medical College and Chang Gung University, Taoyuan, Taiwan.
Jui-Yung YangDepartment of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Chang Gung Medical College and Chang Gung University, Taoyuan, Taiwan.
Oscar K LeeInstitute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
National Yang Ming Chiao Tung University · TWNational Yang Ming University Hospital · TWChang Gung University · TWNational Yang Ming Chiao Tung University · TWChinese University of Hong Kong, Shenzhen · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHypertrophic scars (HSs) are formed via an aberrant response to the wound healing process. HSs can be cosmetic or can result in functional problems. Prolonged proliferation and remodeling phases disrupt wound healing, leading to excessive collagen production and HS formation. However, there are currently no satisfactory drugs to prevent HS formation. Mesenchymal stem cell (MSC) conditioned medium (CM) has therapeutic effects on wound healing and preventing HS formation. Bone marrow concentrate (BMC) contains various growth factors and cytokines that are crucial for regeneration and has been applied in the clinical setting. In this study, we evaluated the effects of BMC-induced MSC CM on HS formation in a rabbit ear model.

methodsWe established a rabbit ear wound model by generating full-thickness wounds in the ears of rabbits (n = 12) and treated wounds with MSC CM, BMC CM, or BMC-induced MSC CM. Dermal fibroblasts from human hypertrophic scar were stimulated with transforming growth factor beta 1 (TGF-β1) for 24 h and cultured in each culture medium for 72 h. We measured the hypertrophic scar (HS) formation during the skin regeneration by measuring the expression of several remodeling molecules and the effect of these conditioned media on active human HS fibroblasts.

resultsOur results showed that BMC-induced MSC CM had greater antifibrotic effects than MSC CM and BMC CM significantly attenuated HS formation in rabbits. BMC-induced MSC CM accelerated wound re-epithelization by increasing cell proliferation. Additionally, BMC-induced MSC CM also inhibited fibrosis by decreasing profibrotic gene and protein expression, promoting extracellular matrix turnover, inhibiting fibroblast contraction, and reversing myofibroblast activation.

conclusionsBMC-induced MSC CM modulated the proliferation and remodeling phases of wound healing, representing a potential wound healing agent and approach for preventing HS formation.

Indexed as

AnimalsBone MarrowCell ProliferationCicatrix, HypertrophicCulture Media, ConditionedEarExtracellular MatrixFemaleFibroblastsMesenchymal Stem CellsMiceMice, Inbred BALB CMyofibroblastsRabbitsSkinTransforming Growth Factor beta1Culture Media, ConditionedTransforming Growth Factor beta1Bone marrow concentrateHypertrophic scarMesenchymal stem cell-conditioned medium

Identifiers

PMID31462299
PMCPMC6714083
OpenAlexW2970470890

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.