ReviewInternational journal of molecular sciences2019
Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification.
Review in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
69 citing papers in PubMed.
- Affinity-Activated Protein Switches for Modular and Signal-ON Detection of Small Molecules.Analytical chemistry · 2026Article
- Genetically encoded tools for tracking metabolites in live cells.Biochemical Society transactions · 2026Review
- Protein Surface Site Determines the Evolutionary Accessibility of Allosteric Regulation.bioRxiv : the preprint server for biology · 2026Article
- Development and application of G4-Flame as a visual biosensor for G4-DNA.Nucleic acids research · 2026Article
- Proteomic sensors for quantitative multiplexed and spatial monitoring of kinase signaling.Nature communications · 2025Article
- Genetically encoded fluorescent probes to assist in the diagnosis of small cell lung cancer.Applied microbiology and biotechnology · 2025Article
- PASTa: An Open-Source Analysis and Signal Processing Toolbox for Fiber Photometry Data.Current protocols · 2025Article
- Characterization and structural basis for the brightness of mCLIFY: A novel monomeric and circularly permuted bright yellow fluorescent protein.Biophysical journal · 2025Article
- Mechanical Modulation, Physiological Roles, and Imaging Innovations of Intercellular Calcium Waves in Living Systems.Cancers · 2025Review
- Protein Engineering for Spatiotemporally Resolved Cellular Monitoring.Annual review of analytical chemistry (Palo Alto, Calif.) · 2025Review
- Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology.Journal of microscopy · 2025Review
- An optical BOD biosensor based on intracellular ATP measurements in genetically modified Saccharomyces cerevisiae.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- A novel protein for bioremediation of gadolinium waste.Protein science : a publication of the Protein Society · 2025Article
- A Yeast-Based Assay for Inhibitors of l-Lactate Transport Utilizing Fluorescent Biosensors.ChemMedChem · 2025Article
- Conditional Control of Benzylguanine Reaction with the Self-Labeling SNAP-tag Protein.Bioconjugate chemistry · 2025Article
- A genetically encoded fluorescent biosensor for visualization of acetyl-CoA in live cells.Cell chemical biology · 2025Article
- Detection of circular permutations by Protein Language Models.Computational and structural biotechnology journal · 2025Article
- Genetically-encoded temperature indicators for thermal biology.Biophysics and physicobiology · 2025Article
- Affinity Peptide-Based Circularly Permuted Fluorescent Protein Biosensors for Non-Small Cell Lung Cancer Diagnosis.Sensors (Basel, Switzerland) · 2024Article
- Biosensing strategies using recombinant luminescent proteins and their use for food and environmental analysis.Analytical and bioanalytical chemistry · 2024Review
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Genetically encoded biosensors based on fluorescent proteins (FPs) are a reliable tool for studying the various biological processes in living systems. The circular permutation of single FPs led to the development of an extensive class of biosensors that allow the monitoring of many intracellular events. In circularly permuted FPs (cpFPs), the original N- and C-termini are fused using a peptide linker, while new termini are formed near the chromophore. Such a structure imparts greater mobility to the FP than that of the native variant, allowing greater lability of the spectral characteristics. One of the common principles of creating genetically encoded biosensors is based on the integration of a cpFP into a flexible region of a sensory domain or between two interacting domains, which are selected according to certain characteristics. Conformational rearrangements of the sensory domain associated with ligand interaction or changes in the cellular parameter are transferred to the cpFP, changing the chromophore environment. In this review, we highlight the basic principles of such sensors, the history of their creation, and a complete classification of the available biosensors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.