ArticleCell reports2019
CRISPR-Cas9 Gene Editing in Lizards through Microinjection of Unfertilized Oocytes.
Article in Cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it, 123 citations in OpenAlex.
- A lizard is never late: Squamate genomics as a recent catalyst for understanding sex chromosome and microchromosome evolution.The Journal of heredity · 2023Pooled it
- The Visual Ecology ofAnimals : an open access journal from MDPI · 2026Review
- A Surgical Method for Oocyte Injection and CRISPR-Cas9 Mutagenesis inCold Spring Harbor protocols · 2026Article
- Post-Oviposition Embryonic Staging Scheme for the Delicate Skink, Lampropholis delicata.Journal of morphology · 2026Article
- Article
- Pre-oviposition development of the brown anole (Anolis sagrei).Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Oogenesis and germinal bed morphology of the brown anole (A. sagrei).Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Poly(A) probe HCR RNA-FISH specifically marks pyriform nurse cells in the brown anole lizard ovary.Developmental biology · 2026Article
- Oogenesis and germinal bed morphology of the brown anole (bioRxiv : the preprint server for biology · 2025Article
- Genomic evolution of EGF-CFC genes in deuterostomes.Developmental dynamics : an official publication of the American Association of Anatomists · 2025Article
- Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo.Nature communications · 2025Article
- Histological analysis of retinal development and remodeling in the brown anole lizard (Anolis sagrei).Journal of anatomy · 2025Article
- CRISPR-Cas9 in the Tailoring of Genetically Engineered Animals.Current issues in molecular biology · 2025Review
- Probe Sequencing Analysis of Regenerating Lizard Tails Indicates Crosstalk Among Osteoclasts, Epidermal Cells, and Fibroblasts.Journal of developmental biology · 2025Article
- The mosaicism of Cas-induced mutations and pleiotropic effects of scarlet gene in an emerging model system.Heredity · 2025Article
- Methodological and Ethical Considerations in the Use of Chordate Embryos in Biomedical Research.International journal of molecular sciences · 2025Review
- Simulation of adult limb regeneration with lizard tail spinal cord implants reveals distinct roles of radial glia and microglia populations.Research square · 2025Article
- High-quality genome assembly and annotation of the crested gecko (Correlophus ciliatus).G3 (Bethesda, Md.) · 2025Article
- In vitro organ culture protocol for intact urogenital systems supporting gonadal differentiation.PloS one · 2025Article
- Molecular insights into foveal hypoplasia: development, genetics, mechanisms, and models.Molecular vision · 2025Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
CRISPR-Cas9-mediated gene editing has enabled the direct manipulation of gene function in many species. However, the reproductive biology of reptiles presents unique barriers for the use of this technology, and there are no reptiles with effective methods for targeted mutagenesis. Here, we demonstrate that the microinjection of immature oocytes within the ovaries of Anolis sagrei females enables the production of CRISPR-Cas9-induced mutations. This method is capable of producing F0 embryos and hatchlings with monoallelic or biallelic mutations. We demonstrate that these mutations can be transmitted through the germline to establish genetically modified strains of lizards. Direct tests of gene function can now be performed in Anolis lizards, an important model for studies of reptile evolution and development.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.