ArticlePLoS pathogens2019
Members of chitin synthase family in Metarhizium acridum differentially affect fungal growth, stress tolerances, cell wall integrity and virulence.
Article in PLoS pathogens, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 75 citations in OpenAlex.
- The role of fungal chitinases in the tripartite interactions among insects, plants and entomopathogenic fungi.Pest management science · 2026Review
- Disruption of MaPigA, a phosphatidylinositol-N-acetylglucosaminyltransferase subunit gene, promotes cuticle penetration and immune evasion through enhancing cell wall α-1,3-glucan in Metarhizium anisopliae.Pest management science · 2026Article
- Genome sequencing and functional annotation of Metarhizium anisopliae TNAU-MA-GDU: Insights into virulence mechanisms against Spodoptera frugiperda.Molecular biology reports · 2026Article
- Article
- Basidiomycete-specific chitin synthase genes have clade-specific roles in cell wall formation and hyphal development inMicrobiology (Reading, England) · 2026Article
- Article
- Oleic Acid and Linoleic Acid Enhances the Biocontrol Potential ofJournal of fungi (Basel, Switzerland) · 2024Article
- Glyoxal oxidase-mediated detoxification of reactive carbonyl species contributes to virulence, stress tolerance, and development in a pathogenic fungus.PLoS pathogens · 2024Article
- Morphological Engineering of Filamentous Fungi: Research Progress and Perspectives.Journal of microbiology and biotechnology · 2024Review
- Integrated Transcriptomics and Metabolomics Analysis Reveal the Regulatory Mechanisms Underlying Sodium Butyrate-Induced Carotenoid Biosynthesis inJournal of fungi (Basel, Switzerland) · 2024Article
- Article
- ChsA, a Class Ⅱ Chitin Synthase, Contributes to Asexual Conidiation, Mycelial Morphology, Cell Wall Integrity, and the Production of Enzymes and Organic Acids inJournal of fungi (Basel, Switzerland) · 2023Article
- Tetracarboxylic acid transporter regulates growth, conidiation, and carbon utilization in Metarhizium acridum.Applied microbiology and biotechnology · 2023Article
- Recent Advances in Chitin Biosynthesis Associated with the Morphology and Secondary Metabolite Synthesis of Filamentous Fungi in Submerged Fermentation.Journal of fungi (Basel, Switzerland) · 2023Review
- A life-and-death struggle: interaction of insects with entomopathogenic fungi across various infection stages.Frontiers in immunology · 2023Review
- MaCts1, an Endochitinase, Is Involved in Conidial Germination, Conidial Yield, Stress Tolerances and Microcycle Conidiation inBiology · 2022Article
- Role of MicroRNA-Like RNAs in the Regulation of Spore Morphological Differences in the Entomopathogenic FungusPolish journal of microbiology · 2022Article
- Disruption of the Chitin Biosynthetic Pathway Results in Significant Changes in the Cell Growth Phenotypes and Biosynthesis of Secondary Metabolites ofJournal of fungi (Basel, Switzerland) · 2022Article
- Virulence-Associated Genes ofJournal of fungi (Basel, Switzerland) · 2022Article
- Identification of Nematicidal Metabolites fromMicroorganisms · 2022Article
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitin is an important component of the fungal cell wall with a family of chitin synthases mediating its synthesis. Here, we report on the genetic characterization of the full suite of seven chitin synthases (MaChsI-VII) identified in the insect pathogenic fungus, Metarhizium acridum. Aberrant distribution of chitin was most evident in targeted gene knockouts of MaChsV and MaChsVII. Mutants of MaChsI, MaChsIII, MaChsIV showed delayed conidial germination, whereas ΔMaChsII and ΔMaChsV mutants germinated more rapidly when compared to the wild-type parent. All MaChs genes impacted conidial yield, but differentially affected stress tolerances. Inactivation of MaChsIII, MaChsV, MaChsVII resulted in cell wall fragility, and ΔMaChsV and ΔMaChsVII mutants showed high sensitivity to Congo red and calcofluor white, suggesting that the three genes are required for cell wall integrity. In addition, ΔMaChsIII and ΔMaChsVII mutants showed the highest sensitivities to heat and UV-B stress. Three of seven chitin synthase genes, MaChsIII, MaChsV, MaChsVII, were found to contribute to fungal virulence. Compared with the wild-type strain, ΔMaChsIII and ΔMaChsV mutants were reduced in virulence by topical inoculation, while the ΔMaChsVII mutant showed more severe virulence defects. Inactivation of MaChsIII, MaChsV, or MaChsVII impaired appressorium formation, affected growth of in insecta produced hyphal bodies, and altered the surface properties of conidia and hyphal bodies, resulting in defects in the ability of the mutant strains to evade insect immune responses. These data provide important links between the physiology of the cell wall and the ability of the fungus to parasitize insects and reveal differential functional consequences of the chitin synthase family in M. acridum growth, stress tolerances, cell wall integrity and virulence.
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