Evidence map›Paper›PMID 31461020›Full record

ArticlePlastic and reconstructive surgery2019

Adipose-Derived Stem Cells and Ceiling Culture-Derived Preadipocytes Cultured from Subcutaneous Fat Tissue Differ in Their Epigenetic Characteristics and Osteogenic Potential.

Yoshitaro Sasahara, Yoshitaka Kubota, Kentaro Kosaka, Naoki Adachi, Yoshihisa Yamaji, Hidekazu Nagano, Shinsuke Akita, Masayuki Kuroda, Tomoaki Tanaka, Hideaki Bujo and 1 more

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Article in Plastic and reconstructive surgery, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Yoshitaro SasaharaFrom the Departments of Plastic Surgery and Molecular Diagnosis and the Center for Advanced Medicine, Chiba University; the Department of General Surgery, National Organization Hospital Mito Medical Center; and the Department of Research and Development, Toho University.
Yoshitaka KubotaFrom the Departments of Plastic Surgery and Molecular Diagnosis and the Center for Advanced Medicine, Chiba University; the Department of General Surgery, National Organization Hospital Mito Medical Center; and the Department of Research and Development, Toho University.
Kentaro KosakaFrom the Departments of Plastic Surgery and Molecular Diagnosis and the Center for Advanced Medicine, Chiba University; the Department of General Surgery, National Organization Hospital Mito Medical Center; and the Department of Research and Development, Toho University.
Naoki AdachiFrom the Departments of Plastic Surgery and Molecular Diagnosis and the Center for Advanced Medicine, Chiba University; the Department of General Surgery, National Organization Hospital Mito Medical Center; and the Department of Research and Development, Toho University.
Yoshihisa YamajiFrom the Departments of Plastic Surgery and Molecular Diagnosis and the Center for Advanced Medicine, Chiba University; the Department of General Surgery, National Organization Hospital Mito Medical Center; and the Department of Research and Development, Toho University.
Hidekazu NaganoFrom the Departments of Plastic Surgery and Molecular Diagnosis and the Center for Advanced Medicine, Chiba University; the Department of General Surgery, National Organization Hospital Mito Medical Center; and the Department of Research and Development, Toho University.
Shinsuke AkitaFrom the Departments of Plastic Surgery and Molecular Diagnosis and the Center for Advanced Medicine, Chiba University; the Department of General Surgery, National Organization Hospital Mito Medical Center; and the Department of Research and Development, Toho University.
Masayuki KurodaFrom the Departments of Plastic Surgery and Molecular Diagnosis and the Center for Advanced Medicine, Chiba University; the Department of General Surgery, National Organization Hospital Mito Medical Center; and the Department of Research and Development, Toho University.
Tomoaki TanakaFrom the Departments of Plastic Surgery and Molecular Diagnosis and the Center for Advanced Medicine, Chiba University; the Department of General Surgery, National Organization Hospital Mito Medical Center; and the Department of Research and Development, Toho University.
Hideaki BujoFrom the Departments of Plastic Surgery and Molecular Diagnosis and the Center for Advanced Medicine, Chiba University; the Department of General Surgery, National Organization Hospital Mito Medical Center; and the Department of Research and Development, Toho University.
Nobuyuki MitsukawaFrom the Departments of Plastic Surgery and Molecular Diagnosis and the Center for Advanced Medicine, Chiba University; the Department of General Surgery, National Organization Hospital Mito Medical Center; and the Department of Research and Development, Toho University.
National Hospital Organization Mito Medical Center · JPIbaraki University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAdipose-derived stem cells and ceiling culture-derived preadipocytes can be harvested from subcutaneous adipose tissue. Little is known about the epigenetic differences, which may contribute to differences in osteogenic potential, between these cell types. The purpose of this study was to address the osteogenic potential and underlying epigenetic status of adipose-derived stem cells and ceiling culture-derived preadipocytes.

methodsAdipose-derived stem cells and ceiling culture-derived preadipocytes were cultured from abdominal subcutaneous fat tissues of four metabolically healthy, lean female patients. After 7 weeks of culture, cellular responses to osteogenic differentiation media were examined. To evaluate the osteogenic potentials of undifferentiated adipose-derived stem cells and ceiling culture-derived preadipocytes, two types of epigenetic assessment were performed using next-generation sequencing: DNA methylation assays with the Human Methylation 450K BeadChip, and chromatin immunoprecipitation assays for trimethylation of histone H3 at lysine 4.

resultsHuman ceiling culture-derived preadipocytes showed greater osteogenic differentiation ability than did adipose-derived stem cells. In an epigenetic survey of the promoters of four osteogenic regulator genes (RUNX2, SP7, ATF4, and BGLAP), the authors found a general trend toward decreased CpG methylation and increased trimethylation of histone H3 at lysine 4 levels in ceiling culture-derived preadipocytes as compared to adipose-derived stem cells, indicating that these genes were more likely to be highly expressed in ceiling culture-derived preadipocytes.

conclusionsThe surveyed epigenetic differences between adipose-derived stem cells and ceiling culture-derived preadipocytes were consistent with the observed differences in osteogenic potential. These results enhance the authors' understanding of these cells and will facilitate their further application in regenerative medicine.

Indexed as

AdipocytesAdultCells, CulturedEpigenesis, GeneticFemaleHumansMiddle AgedOsteogenesisStem CellsSubcutaneous Fat

Identifiers

PMID31461020
OpenAlexW2951265010

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.