ArticleAnalytical chemistry2019
Targeted Quantitative Proteomic Approach for High-Throughput Quantitative Profiling of Small GTPases in Brain Tissues of Alzheimer's Disease Patients.
Article in Analytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- Targeted Proteomic Profiling Revealed Roles of Small GTPases during Osteogenic Differentiation.Analytical chemistry · 2023Article
- Targeted Proteomic Analysis of Small GTPases in Radioresistant Breast Cancer Cells.Analytical chemistry · 2022Article
- GLOBAL AND TARGETED PROFILING OF GTP-BINDING PROTEINS IN BIOLOGICAL SAMPLES BY MASS SPECTROMETRY.Mass spectrometry reviews · 2021Review
- Proteome-wide Interrogation of Small GTPases Regulated byAnalytical chemistry · 2020Article
- Targeted Proteomic Analysis of Small GTPases in Murine Adipogenesis.Analytical chemistry · 2020Article
- Oncogenic effects of RAB27B through exosome independent function in renal cell carcinoma including sunitinib-resistant.PloS one · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Neurodegenerative disorders, including Alzheimer's disease (AD), are prevalent among the elderly. Small GTPases of the Ras superfamily are essential regulators of intracellular trafficking and signal transduction. In this study, we develop a targeted quantification method for small GTPase proteins, where the method involves scheduled multiple-reaction monitoring analysis and the use of synthetic stable isotope-labeled peptides as internal standards or surrogate standards. We further applied this method to examine the altered expression of small GTPase proteins in post-mortem frontal cortex tissues from AD patients with different degrees of disease severity. We were able to achieve sensitive and reproducible quantifications of 80 small GTPases in brain tissue samples from 15 patients. Our results revealed substantial up-regulations of several synaptic GTPases, i.e., RAB3A/C, RAB4A/B, and RAB27B, in tissues from patients with higher degrees of AD pathology, suggesting that aberrant synaptic trafficking may modulate the progression of AD. The method should be generally applicable for high-throughput targeted quantification of small GTPase proteins in other tissue and cellular samples.
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Registered trials
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