Evidence map›Paper›PMID 31460473›Full record

ArticleACS omega2019

Bioconjugation with Aminoalkylhydrazine for Efficient Mass Spectrometry-Based Detection of Small Carbonyl Compounds.

Senthil K Thangaraj, Sanni Voutilainen, Martina Andberg, Anu Koivula, Janne Jänis, Juha Rouvinen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Senthil K ThangarajDepartment of Chemistry, University of Eastern Finland, PO Box 111, FI-80101 Joensuu, Finland.
Sanni VoutilainenVTT Technical Research Centre of Finland Ltd, PO Box 1000, FI-020444 VTT, Espoo, Finland.
Martina AndbergVTT Technical Research Centre of Finland Ltd, PO Box 1000, FI-020444 VTT, Espoo, Finland.
Anu KoivulaVTT Technical Research Centre of Finland Ltd, PO Box 1000, FI-020444 VTT, Espoo, Finland.
Janne JänisDepartment of Chemistry, University of Eastern Finland, PO Box 111, FI-80101 Joensuu, Finland.
Juha RouvinenDepartment of Chemistry, University of Eastern Finland, PO Box 111, FI-80101 Joensuu, Finland.
University of Eastern Finland · FIVTT Technical Research Centre of Finland · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bioconjugation through oxime or hydrazone formation is a versatile strategy for covalent labeling of biomolecules in vitro and in vivo. In this work, a mass spectrometry-based method was developed for the bioconjugation of small carbonyl compounds (CCs) with an aminoalkylhydrazine to form stable hydrazone conjugates that are readily detectable with electrospray ionization mass spectrometry (ESI-MS). Out of all hydrazine reagents tested, 2-(dimethylamino)ethylhydrazine (DMAEH) was selected for further analysis due to the fastest reaction rates observed. A thorough study of the reaction kinetics between structurally varied short-chain CCs and DMAEH was performed with the second-order reaction rate constants spanning in the range of 0.23-208 M

Identifiers

PMID31460473
PMCPMC6705233
OpenAlexW2965045825

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.