Evidence map›Paper›PMID 31460466›Full record

ArticleACS omega2019

Systematic Probing of the Sequence Selectivity of Exonuclease III with a Photosensitization Colorimetric Assay.

Yu Ding, Xianming Li, Xinfeng Zhang, Feng Li, Xiandeng Hou, Peng Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yu DingAnalytical & Testing Center, College of Chemistry, Sichuan University, Chengdu 610064, China.
Xianming LiAnalytical & Testing Center, College of Chemistry, Sichuan University, Chengdu 610064, China.
Xinfeng ZhangCollege of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China.
Feng LiAnalytical & Testing Center, College of Chemistry, Sichuan University, Chengdu 610064, China.
Xiandeng HouAnalytical & Testing Center, College of Chemistry, Sichuan University, Chengdu 610064, China.
Peng WuAnalytical & Testing Center, College of Chemistry, Sichuan University, Chengdu 610064, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exonuclease III (Exo III) is an important enzymatic tool that is being widely used in molecular biology, biotechnology, and bioassay development. Exo III prefers to cleave double-stranded DNA (dsDNA) with blunt and recessed 3'-termini rather than their protruding counterpart. While it has been accepted that a short 3'-overhang (e.g., >4 nt) is necessary to protect a dsDNA from Exo III cleavage, critical roles of the length and sequence of this 3'-overhang remain unexplored. Herein, we develop a novel light-induced colorimetric assay allowing the systematic probe of the sequence selectivity of Exo III in a rapid and high-throughput manner. Our finding that Exo III is highly specific to 3'-overhang in terms of both length and sequence will be valuable for guiding the design of bioassays and DNA manipulating tools mediated by Exo III.

Identifiers

PMID31460466
PMCPMC6705212

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.