ArticleACS omega2019
Facile and Label-Free Electrochemical Biosensors for MicroRNA Detection Based on DNA Origami Nanostructures.
Article in ACS omega, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- DNA Nanoarray for Multiplexed RNA Detection With Single-Molecule Readout.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Comprehensive Review on DNA Hydrogels and DNA Origami-Enabled Wearable and Implantable Biosensors.Biosensors · 2025Review
- DNA Nanostructure Deposition on Self-Assembled Monolayers.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- An electrochemical biosensor for the detection of microRNA-31 as a potential oral cancer biomarker.Journal of biological engineering · 2025Article
- Modular DNA origami-based electrochemical detection of DNA and proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Establishment of an amplification strategy - specific binding - convenient processing integrated aflatoxin B1 detection method based on FeFood chemistry: X · 2024Article
- Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead.ACS sensors · 2023Review
- Article
- Advances in Electrochemical Biosensor Technologies for the Detection of Nucleic Acid Breast Cancer Biomarkers.Sensors (Basel, Switzerland) · 2023Review
- Non-Viral Carriers for Nucleic Acids Delivery: Fundamentals and Current Applications.Life (Basel, Switzerland) · 2023Review
- Label-Free Electrochemical Biosensor Platforms for Cancer Diagnosis: Recent Achievements and Challenges.Biosensors · 2023Review
- Article
- Self-assembled endogenous DNA nanoparticles for auto-release and expression of the eGFP gene in Bacillus subtilis.Communications biology · 2022Article
- Dual-mode sensor based on the synergy of magnetic separation and functionalized probes for the ultrasensitive detection ofRSC advances · 2022Article
- DNA-Based Biosensors for the Biochemical Analysis: A Review.Biosensors · 2022Review
- Extracellular Biomarkers of Inner Ear Disease and Their Potential for Point-of-Care Diagnostics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Carbon dots hybrid for dual fluorescent detection of microRNA-21 integrated bioimaging of MCF-7 using a microfluidic platform.Journal of nanobiotechnology · 2022Article
- Evolution of nucleic acids biosensors detection limit III.Analytical and bioanalytical chemistry · 2022Review
- Probing the Conformational States of a pH-Sensitive DNA Origami Zipper via Label-Free Electrochemical Methods.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- miR-486-5p: A Prognostic Biomarker for Chronic Myeloid Leukemia.ACS omega · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) have emerged as the promising molecular biomarkers for early diagnosis and enhanced understanding of the molecular pathogenesis of cancers as well as certain diseases. Here, a facile, label-free, and amplification-free electrochemical biosensor was developed to detect miRNA by using DNA origami nanostructure-supported DNA probes, with methylene blue (MB) serving as the hybridization redox indicator, for the first time. Specifically, the use of cross-shaped DNA origami nanostructures containing multiple single-stranded DNA probes at preselected locations on each DNA nanostructure could increase the accessibility and the recognition efficiency of the probes (due to the rational controlled density of DNA probes). The successful immobilization of DNA origami probes and their hybridization with targeted miRNA-21 molecules was confirmed by electrochemical impedance spectroscopy and cyclic voltammetry methods. A differential pulse voltammetry technique was employed to record the oxidation peak current of MB before and after target hybridization. The linear detection range of this biosensor was from 0.1 pM to 10.0 nM, with a lower detection limit of 79.8 fM. The selectivity of the miRNA biosensor was also studied by observing the discrimination ability of single-base mismatched sequences. Because of the larger surface area and unprecedented customizability of DNA origami nanostructures, this strategy demonstrated great potential for sensitive, selective, and label-free determination of miRNA for translational biomedical research and clinical applications.
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Registered trials
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