ArticleBioprinting (Amsterdam, Netherlands)2019
Scaffold-free Bioprinting of Mesenchymal Stem Cells with the Regenova Printer: Optimization of Printing Parameters.
Article in Bioprinting (Amsterdam, Netherlands), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Engineering Functional Cardiac Tissues for Regenerative Medicine Applications.Current cardiology reports · 2019Pooled it
- Advances in 3D Bioprinting for Scaffold-Based and Scaffold-Free Tissue Engineering and Regenerative Medicine.Gels (Basel, Switzerland) · 2026Review
- Challenges and opportunities in generating microvasculature using bioprinting techniques.npj biomedical innovations · 2026Review
- From microtissues to macro solutions - The future of scalable and automated cartilage tissue engineering.Journal of orthopaedic translation · 2026Review
- Force-sensing mobile microrobotic grippers for gentle and precise bioassembly of cell spheroids.APL bioengineering · 2026Article
- Aspiration-assisted bioprinting of spheroids.Nature protocols · 2026Review
- Bioengineering of Periodontal Tissues: Cell Therapy and Biomaterials Application.Bioengineering (Basel, Switzerland) · 2025Review
- 3D bioprinting in tissue engineering: current state-of-the-art and challenges towards system standardization and clinical translation.Biofabrication · 2025Review
- 3D bioprinting technology innovation in female reproductive system.Materials today. Bio · 2025Review
- Computational methods for biofabrication in tissue engineering and regenerative medicine - a literature review.Computational and structural biotechnology journal · 2024Review
- High-throughput bioprinting of spheroids for scalable tissue fabrication.Nature communications · 2024Article
- Scaffold-free bone-like 3D structure established through osteogenic differentiation from human gingiva-derived stem cells.Biochemistry and biophysics reports · 2024Article
- Advancing cardiac regeneration through 3D bioprinting: methods, applications, and future directions.Heart failure reviews · 2024Review
- Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications.Advanced drug delivery reviews · 2024Review
- Robotics-Driven Manufacturing of Cartilaginous Microtissues for Skeletal Tissue Engineering Applications.Stem cells translational medicine · 2024Article
- Advances in 3D Bioprinting: Techniques, Applications, and Future Directions for Cardiac Tissue Engineering.Bioengineering (Basel, Switzerland) · 2023Review
- Engineering three-dimensional bone macro-tissues by guided fusion of cell spheroids.Frontiers in endocrinology · 2023Article
- Current Advances of Three-Dimensional Bioprinting Application in Dentistry: A Scoping Review.Materials (Basel, Switzerland) · 2022Article
- Additive Manufactured Polymers in Dentistry, Current State-of-the-Art and Future Perspectives-A Review.Polymers · 2022Review
- Bioink Formulation and Machine Learning-Empowered Bioprinting Optimization.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The Kenzan bioprinting method provides a high-resolution biofabrication process by facilitating the fusion of submillimeter cell aggregates (spheroids) into larger tissue constructs on a needle array that is removed upon spheroid fusion. Although the method is relatively straightforward in principle, Kenzan method bioprinting relies on a complex 3D bioprinter (Regenova Bio 3D Printer, Cyfuse, K.K., Japan) implementing an advanced vision system to verify the microscopic spheroids' geometry and high-precision mechatronics to aseptically manipulate the spheroids into position. Due to the complexity of the operation, the need for aseptic conditions, and the size of the spheroids, proficiency with the Regenova Bio 3D Printer and the Kenzan method requires development of best practices and troubleshooting techniques to ensure a robust print and minimize the use of resources. In addition, managing the construct post-bioprinting both in culture and for surgical implantation requires careful consideration and workflow design. Here, we describe methods for generating a competent tissue construct and optimizing the bioprinting process. Optimization resulted in a 4-fold reduction in print times, a 20-fold reduction in the use of bioprinting nozzles, and more robust constructs. The results and procedures described herein will have potential applications for tissue engineering, research, and clinical uses in the future.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.