ArticleScience advances2019
Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array.
Article in Science advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Review
- Intrinsically disordered protein droplet-enhanced oligonucleotide assembly enables rapid oligonucleotide-to-protein expression.Nucleic acids research · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Rapid Isothermal DNA Amplification in Microchambers Detected by Fluorescence RNA Aptamer Transcription.Diagnostics (Basel, Switzerland) · 2025Article
- Self-growing protocell models in aqueous two-phase system induced by internal DNA replication reaction.Nature communications · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- An Automated Cell-Free Workflow for Transcription Factor Engineering.ACS synthetic biology · 2024Article
- Rheostats, toggles, and neutrals, Oh my! A new framework for understanding how amino acid changes modulate protein function.The Journal of biological chemistry · 2024Review
- Identification of activity-based biomarkers for early-stage pancreatic tumors in blood using single-molecule enzyme activity screening.Cell reports methods · 2024Article
- Cell-free protein synthesis with technical additives - expanding the parameter space of in vitro gene expression.Beilstein journal of organic chemistry · 2024Article
- A sequential one-pot approach for rapid and convenient characterization of putative restriction-modification systems.mSystems · 2023Article
- Digital Cascade Assays for ADP- or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device.ACS sensors · 2023Article
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- A biological camera that captures and stores images directly into DNA.Nature communications · 2023Article
- Applications of synthetic biology in medical and pharmaceutical fields.Signal transduction and targeted therapy · 2023Review
- Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles.ACS synthetic biology · 2023Article
- Genetic Perturbation Alters Functional Substates in Alkaline Phosphatase.Journal of the American Chemical Society · 2023Article
- Automating the High-Throughput Screening of Protein-Based Optical Indicators and Actuators.Biochemistry · 2023Review
- Emerging platforms for high-throughput enzymatic bioassays.Trends in biotechnology · 2023Review
- High-throughput screening of cell-free riboswitches by fluorescence-activated droplet sorting.Nucleic acids research · 2022Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a general strategy based on digital counting principle that enables an efficient acquisition of enzyme mutants with desired activities from just a few clones within a day. We prepared a high-density femtoliter droplet array, consisting of 1 million uniform droplets per 1 cm
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.