Evidence map›Paper›PMID 31457061›Full record

ArticleIranian journal of biotechnology2019

Enhancement of Soluble Expression and Biochemical Characterization of Two Epoxide Hydrolases from

Li-Ying Wu, Jun-Jie Xu, Pan Xu, Bin Yong, Hong Feng

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Article in Iranian journal of biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Li-Ying WuKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University; Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610065, Sichuan, P. R. China.
Jun-Jie XuKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University; Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610065, Sichuan, P. R. China.
Pan XuCollege of Life Sciences, Sichuan Normal University, Chengdu 610101, Sichuan, P. R. China.
Bin YongCollege of Life Sciences, Sichuan Normal University, Chengdu 610101, Sichuan, P. R. China.
Hong FengKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University; Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610065, Sichuan, P. R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEnantiopure epoxides are important intermediates in the synthesis of high-value chiral chemicals. Epoxide hydrolases have been exploited in biocatalysis for kinetic resolution of racemic epoxides to produce enantiopure epoxides and vicinal diols. It is necessary to obtain sufficient stable epoxide hydrolases with high enantioselectivity to meet the requirements of industry.

objectivesEnhancement of soluble expression and biochemical characterization of epoxide hydrolases from MATERIAL AND

methodsHomologous genes encoding epoxide hydrolases from

resultsLow temperature induction of expression and a C-terminal-fused His-tag enhanced soluble expression of the epoxide hydrolases from the two

conclusionsThe position of the His-tag and the induction temperature were found to play a vital role in soluble expression of these two epoxide hydrolases in

Indexed as

BacillusEpoxide HydrolaseHydrolysis

Identifiers

PMID31457061
PMCPMC6697846

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