ArticleIranian journal of biotechnology2019
Genetic Transformation of Oat Mediated by
Article in Iranian journal of biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The trial behind it
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Who cites it
3 citing papers in PubMed.
- Conquering Limitations: Exploring the Factors that Drive Successful Agrobacterium-Mediated Genetic Transformation of Recalcitrant Plant Species.Molecular biotechnology · 2025Review
- From Petri Dish to Field: Plant Tissue Culture and Genetic Engineering of Oats for Improved Agricultural Outcomes.Plants (Basel, Switzerland) · 2023Review
- Optimisation of root traits to provide enhanced ecosystem services in agricultural systems: A focus on cover crops.Plant, cell & environment · 2022Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOat (
objectivesThe current study aimed at achieving a standardized protocol for Agrobacterium-mediated transformation in oat. MATERIALS AND
methodsFor oat transformation, mature seeds were sterilized, germinated, and used for explants generation.
resultsAmong the different transformation treatments, the vacuum treatment with 72 hours dark incubation had the best results. Vacuum infiltration of the cultures from leaf base produced a maximum of 25% hygromycin-resistant explants. These explants upon GUS assay and PCR analysis revealed 21.85% and 19.04% transformation efficiency, respectively.
conclusionsIt could be concluded that vacuum infiltration assisted Agrobacterium-mediated transformation is the most efficient method to conduct the genetic improvement of the oat using transformation protocol.
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Registered trials
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