Evidence map›Paper›PMID 31456171›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2019

CelB is a suitable marker for rapid and specific identification of Klebsiella pneumoniae by the loop-mediated isothermal amplification (LAMP) assay.

Yichen Tian, Lefei Wang, Jinyang Zhang, Qinqin Han, Xue-Shan Xia, Yuzhu Song, Guangying Yang

Open access · bronzeAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Rapid molecular detection of pathogenic microorganisms and antimicrobial resistance markers in blood cultures: evaluation and utility of the next-generation FilmArray Blood Culture Identification 2 panel.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2022
    Article
  3. Article
  4. The ZKIR Assay, a Real-Time PCR Method for the Detection ofApplied and environmental microbiology · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yichen TianFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China.
Lefei WangFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China.
Jinyang ZhangFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China.
Qinqin HanFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China.
Xue-Shan XiaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China.
Yuzhu SongFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China. yuzhusong@kmust.edu.cn.ORCID http://orcid.org/0000-0002-9836-0252
Guangying YangFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China. yguangying@foxmail.com.
Kunming University of Science and Technology · CN

Funding

Yunnan Provincial Science and Technology Department 2015BC001Yunnan Provincial Science and Technology Department 2015DH010
6 · The paper itself

Abstract

Klebsiella pneumoniae belongs to Enterobacteriaceae, which is the commonest bacterium causing nosocomial respiratory tract infection. It ranks second in bacteremia and urinary tract infection in gram-negative bacteria. Therefore, the rapid and accurate identification of K. pneumoniae was of great significance for the guide of clinical medication, and timely treatment of patients. The purpose of this study was to establish a rapid and sensitive molecular detection method for K. pneumoniae based on loop-mediated isothermal amplification (LAMP) technology. Firstly, local BLAST and NCBI BLAST were used to analyze the genome of K. pneumoniae. According to the principle of interspecific and intraspecific specificity, CelB (GenBank ID 11847805) was selected as the specific gene. Then, the LAMP and PCR identification systems were established with this target gene. Thirty-six clinical isolates of K. pneumoniae and 50 non-K. pneumoniae were used for the specific evaluation, and both LAMP and PCR could specifically distinguish K. pneumoniae from non-K. pneumoniae. A 10-fold series diluted positive plasmids and simulated infected blood samples were used as the templates in the sensitivity assay, and the results showed that the sensitivity could reach 1 copy/reaction. In summary, a rapid, specific, and sensitive LAMP method was established to detect K. pneumoniae in clinics.

Indexed as

BacteremiaBacterial ProteinsBacterial Typing TechniquesDNA PrimersHumansKlebsiella InfectionsKlebsiella pneumoniaeNucleic Acid Amplification TechniquesPlasmidsSensitivity and SpecificityBacterial ProteinsDNA PrimersKlebsiella pneumoniaeLoop-mediated isothermal amplificationMolecular detectionPolymerase chain amplification

Identifiers

PMID31456171
PMCPMC6863289
OpenAlexW2970869289

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.