ArticleThe Journal of biological chemistry2019
A whole-cell, high-throughput hydrogenase assay to identify factors that modulate [NiFe]-hydrogenase activity.
Article in The Journal of biological chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- A Gas Chromatography-Based Workflow Leads to the Detection of Hydrogenase Activity in Native Bacteria.Chembiochem : a European journal of chemical biology · 2026Article
- Rational engineering of facultative anaerobiosis enables commensal survival in the oxygenated gut.bioRxiv : the preprint server for biology · 2026Article
- Search for the role of a Huc-type [NiFe]-hydrogenase of the soil thermophileFrontiers in microbiology · 2026Article
- Selective inhibition of NikA mediated Ni(II) import in E. coli by the Indium(III)-EDTA complex.Metallomics : integrated biometal science · 2025Article
- Targeting bacterial nickel transport with aspergillomarasmine A suppresses virulence-associated Ni-dependent enzymes.Nature communications · 2024Article
- Differential Contribution of Hydrogen Metabolism toPathogens (Basel, Switzerland) · 2023Article
- Promising approaches for the assembly of the catalytically active, recombinant Desulfomicrobium baculatum hydrogenase with substitutions at the active site.Microbial cell factories · 2023Article
- Biochemical studies highlight determinants for metal selectivity in the Escherichia coli periplasmic solute binding protein NikA.Metallomics : integrated biometal science · 2022Article
- Deacidification by FhlA-dependent hydrogenase is involved in urease activity and urinary stone formation in uropathogenic Proteus mirabilis.Scientific reports · 2020Article
- Activation of a [NiFe]-hydrogenase-4 isoenzyme by maturation proteases.Microbiology (Reading, England) · 2020Article
- Heterologous Hydrogenase Overproduction Systems for Biotechnology-An Overview.International journal of molecular sciences · 2020Review
- Ynt is the primary nickel import system used by Proteus mirabilis and specifically contributes to fitness by supplying nickel for urease activity.Molecular microbiology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
[NiFe]-hydrogenases have attracted attention as potential therapeutic targets or components of a hydrogen-based economy. [NiFe]-hydrogenase production is a complicated process that requires many associated accessory proteins that supply the requisite cofactors and substrates. Current methods for measuring hydrogenase activity have low throughput and often require specialized conditions and reagents. In this work, we developed a whole-cell high-throughput hydrogenase assay based on the colorimetric reduction of benzyl viologen to explore the biological networks of these enzymes in
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