ArticleMethods in enzymology2019
Monitoring gasdermin pore formation in vitro.
Article in Methods in enzymology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- De novo discovery of bicyclic cysteine-rich peptides targeting gasdermin D.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Proteolytic activation of diverse antiviral defense modules in prokaryotes.bioRxiv : the preprint server for biology · 2025Article
- Mechanisms of RCD-1 pore formation and membrane bending.Nature communications · 2025Article
- Advances of Protein Palmitoylation in Tumor Cell Deaths.Cancers · 2023Review
- Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis.Immunity · 2023Article
- Pyroptosis Modulators: New Insights of Gasdermins in Health and Disease.Antioxidants (Basel, Switzerland) · 2023Review
- Pore-Forming Proteins: From Pore Assembly to Structure by Quantitative Single-Molecule Imaging.International journal of molecular sciences · 2023Review
- NLRP3: a new therapeutic target in alcoholic liver disease.Frontiers in immunology · 2023Review
- Emerging role of STING signalling in CNS injury: inflammation, autophagy, necroptosis, ferroptosis and pyroptosis.Journal of neuroinflammation · 2022Review
- Role of Pyroptosis in Gynecological Oncology and Its Therapeutic Regulation.Biomolecules · 2022Review
- Protective effects of dietary grape against atopic dermatitis-like skin lesions in NC/NgaTndCrlj mice.Frontiers in immunology · 2022Article
- Evaluation of I-TAC as a potential early plasma marker to differentiate between critical and non-critical COVID-19.Cell stress · 2022Article
- Tumor necrosis factor-alpha and interferon-gamma induce inflammasome-mediated corneal endothelial cell death.Experimental eye research · 2021Article
- Article
- Biological Functions of Gasdermins in Cancer: From Molecular Mechanisms to Therapeutic Potential.Frontiers in cell and developmental biology · 2021Review
- Biological mechanisms and therapeutic relevance of the gasdermin family.Molecular aspects of medicine · 2020Review
- A comprehensive guide to studying inflammasome activation and cell death.Nature protocols · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The gasdermin (GSDM) family consists of gasdermin A (GSDMA), B (GSDMB), C (GSDMC), D (GSDMD), E or DNFA5 (GSDME), and DFNB59 in human. Expressed in the skin, gastrointestinal tract, and various immune cells, GSDMs mediate homeostasis and inflammation upon activation by caspases and unknown proteases. In particular, GSDMD is activated by inflammasome-activated caspases-1/-4/-5/-11 as well as a caspase-8-mediated pathway during Yersinia infection. These caspases cleave GSDMD to release its functional N-terminal fragment (GSDMD-NT) from its auto-inhibitory C-terminal fragment (GSDMD-CT). GSDMD-NTs bind to acid lipids in mammalian cell membranes and bacterial membranes, oligomerize, and insert into the membranes to form large transmembrane pores. Consequently, cellular contents including inflammatory cytokines are released and cells can undergo pyroptosis, a highly inflammatory form of cell death. In this chapter, we summarize recent research findings and present experimental procedures to obtain pure recombinant GSDMs for biochemical studies. We highlight a liposome-based assay that yields robust fluorescence signals for characterizing GSDM activities in vitro and may be applicable to other pore-forming proteins and ion channels in general.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.