ArticlePloS one2019
Detection of apoptosis and matrical degeneration within the intervertebral discs of rats due to passive cigarette smoking.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Article
- Smoking, Alcohol Use, and Low Back Pain: A Large-Scale Study Identifying a Synergistic Lifestyle Risk.Global spine journal · 2026Article
- A Comparison of Cigarette Smoking Effects on Intervertebral Disc Cell Metabolism in a Rat Tissue Model.Annals of biomedical engineering · 2026Article
- Engineering Intervertebral Disc Regeneration: Biomaterials, Cell Sources and Animal Models.Cell proliferation · 2025Review
- Hyaluronidase induces degenerative effects on intervertebral endplate cells via upregulation of PTGS2.Cytotechnology · 2025Article
- Analysis of Nicotine Toxicity and Mechanisms of Senescence in Nucleus Pulposus Cells Using Network Toxicology and Molecular Docking Technique.JOR spine · 2025Article
- Endplate remodeling: a key indicator of cigarette smoke exposure-induced intervertebral disc degeneration in a male rat model.JBMR plus · 2025Article
- Effect of cigarette smoke exposure and cessation on regional diffusion properties in rat intervertebral discs.JOR spine · 2024Article
- Consensus statement on smoking cessation in patients with pain.Journal of anesthesia · 2022Review
- Smoking and degenerative spinal disease: A systematic review.Brain & spine · 2022Review
- Canine models of spine disorders.JOR spine · 2020Article
- Review
- Epidemiology of chronic back pain among adults and elderly from Southern Brazil: a cross-sectional study.Brazilian journal of physical therapyArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although low-back pain is considered to be associated with cigarette smoking, the influence of cigarette smoking on the intervertebral discs (IVD) has not been confirmed. We established a rat model of passive cigarette smoking-induced IVD degeneration, and investigated the cytohistological changes in the IVD and the accompanying changes in gene expression. IVD from rats exposed to 8 weeks of passive cigarette smoking were stained with Elastica van Gieson, and exhibited marked destruction of the supportive structure of the reticular matrix in the nucleus pulposus (NP). Positive signals on safranin O, alcian blue, type II collagen and aggrecan staining were decreased in the destroyed structure. Safranin O and type II collagen signals were also decreased in the cartilage end-plate (CEP) after 4- and 8-weeks of cigarette smoking. In the CEP, the potential for apoptosis was increased significantly, as demonstrated by staining for single-strand DNA. However, there were no signs of apoptosis in the NP or annulus fibrosus cells. Based on these findings, we hypothesized that passive cigarette smoking-induced stress stimuli first affect the CEP through blood flow due to the histological proximity, thereby stimulating chondrocyte apoptosis and reduction of the extracellular matrix (ECM). This leads to reduction of the ECM in the NP, destroying the NP matrix, which can then progress to IVD degeneration.
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