Evidence map›Paper›PMID 31454137›Full record

ArticleCellular microbiology2019

An essential pentatricopeptide repeat protein in the apicomplexan remnant chloroplast.

Joanna L Hicks, Imen Lassadi, Emma F Carpenter, Madeleine Eno, Alexandros Vardakis, Ross F Waller, Christopher J Howe, R Ellen R Nisbet

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Essential Genes of the Parasitic Apicomplexa.Trends in parasitology · 2021
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 2 countries.

Joanna L HicksDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Imen LassadiDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Emma F CarpenterDepartment of Biochemistry, University of Cambridge, Cambridge, UK.ORCID 0000-0002-1911-6842
Madeleine EnoDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Alexandros VardakisDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Ross F WallerDepartment of Biochemistry, University of Cambridge, Cambridge, UK.ORCID 0000-0001-6961-9344
Christopher J HoweDepartment of Biochemistry, University of Cambridge, Cambridge, UK.ORCID 0000-0002-6975-8640
R Ellen R NisbetDepartment of Biochemistry, University of Cambridge, Cambridge, UK.ORCID 0000-0003-4487-196X
University of Cambridge · GB

Funding

Medical Research Council MC_PC_14116Medical Research Council MR/M011690/1MRC MR/M011690/1University of CambridgeWellcome TrustWellcome Trust 094249
6 · The paper itself

Abstract

The malaria parasite Plasmodium and other apicomplexans such as Toxoplasma evolved from photosynthetic organisms and contain an essential, remnant plastid termed the apicoplast. Transcription of the apicoplast genome is polycistronic with extensive RNA processing. Yet little is known about the mechanism of apicoplast RNA processing. In plants, chloroplast RNA processing is controlled by multiple pentatricopeptide repeat (PPR) proteins. Here, we identify the single apicoplast PPR protein, PPR1. We show that the protein is essential and that it binds to RNA motifs corresponding with previously characterized processing sites. Additionally, PPR1 shields RNA transcripts from ribonuclease degradation. This is the first characterization of a PPR protein from a nonphotosynthetic plastid.

Indexed as

ApicoplastsChloroplastsPhylogenyPlasmodium falciparumToxoplasmaapicomplexaapicoplastPlasmodiumplastidRNA processingToxoplasma

Identifiers

PMID31454137
PMCPMC6899631
OpenAlexW2970760184

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.