ArticleJournal of biological methods2018
A highly-simplified and inexpensive MALDI-TOF mass spectrometry sample-preparation method with broad applicability to microorganisms, plants, and insects.
Article in Journal of biological methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- An easy adjustment of instrument settings ('Peak MALDI') improves identification of organisms by MALDI-ToF mass spectrometry.Scientific reports · 2023Article
- Spectrometric Characterization of Clinical and Environmental Isolates ofJournal of fungi (Basel, Switzerland) · 2023Article
- Species Identification and Mycotoxigenic Potential ofMicroorganisms · 2023Article
- Article
- Article
- Metrological framework for selecting morphological characters to identify species and estimate developmental maturity of forensically significant insect specimens.Forensic sciences research · 2020Article
- A Polyphasic Approach Aids Early Detection of Potentially Toxigenic Aspergilli in Soil.Microorganisms · 2019Article
- Spectral Grouping of NominallyMicroorganisms · 2019Article
- Discrimination between Asian populations of the parasitoid waspBiology methods & protocols · 2019Article
- Identification ofBiology methods & protocols · 2019Article
- A method for filamentous fungal growth and sample preparation aimed at more consistent MALDI-TOF MS spectra despite variations in growth rates and/or incubation times.Biology methods & protocols · 2019Article
- MALDI-TOF MS-based analysis of dried seed proteins immobilized on filter paper.Biology methods & protocols · 2019Article
- MALDI-TOF MS protein fingerprinting of mixed samples.Biology methods & protocols · 2019Article
- Discrimination between regional biotypes ofPlant methods · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser-desorption and ionization time-of-flight mass spectrometry prepares proteins intact in the gas phase with predominantly a single positive charge. The times-of-flight of charged proteins along a tube held at high vacuum after acceleration in an electrical field are proportional to the square root of the mass-over-charge ratios for the proteins, thereby allowing a mass spectrum to be generated, which can then be used to characterize or identify a protein-containing sample. Several sample-preparation methods are currently available but not all of these are applicable to some forms of fungal biomass and few of these are well suited to the analysis of plant or insect material. We have therefore developed a simplified method that: lyses cells, selectively solubilizes basic proteins, dissolves matrix to a suitable concentration, generates spectra with good intensity and peak richness, costs no more (and generally less) than current methods, and is not constrained in terms of throughput by the availability of centrifuges. Using this method, and a reagent formulation comprising α-cyano-4-hydroxycinnamic acid matrix close to saturation in 60%-65% (v/v) acetonitrile in water containing 2.5% (v/v) trifluoroacetic acid, we have been able to differentiate between strains for a representative subset of aflatoxin-producing and aflatoxin-non-producing strains of
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