Evidence map›Paper›PMID 31453245›Full record

ArticleJournal of biological methods2018

RNA isolation from Peyer's patch lymphocytes and mononuclear phagocytes to determine gene expression profiles using NanoString technology.

Navjot Singh, Heather C Gallagher, Renjie Song, Jaskiran K Dhinsa, Gary R Ostroff, Magdia De Jesus

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biological methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Navjot SinghDivision of Molecular Genetics Wadsworth Center, New York State Department of Health, Albany NY, USA.
Heather C GallagherDivision of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany NY, USA.
Renjie SongBiochemistry and Immunology Core, Wadsworth Center, New York State Department of Health, Albany NY, USA.
Jaskiran K DhinsaDivision of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany NY, USA.
Gary R OstroffProgram in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Magdia De JesusDivision of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany NY, USA.
Wadsworth Center · USNew York State Department of Health · USUniversity of Massachusetts Chan Medical School · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sampling and immune surveillance within gut-associated lymphoid tissues such as the intestinal Peyer's patch (PP) occurs by an elegantly orchestrated effort that involves the epithelial barrier, B and T lymphocytes, and an extensive network of mononuclear phagocytes. Although we now understand more about the dynamics of antigen and microbial sampling within PPs, the gene expression changes that occur in individual cell subsets during sampling are not well characterized. This protocol describes the isolation of high-quality RNA from sorted PP, B and T-lymphocytes, and CD11c

Indexed as

B-lymphocytesmononuclear phagocytesNanoString technologyPeyer’s patchesT-lymphocytes

Identifiers

PMID31453245
PMCPMC6706143
OpenAlexW2871774252

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.