Evidence map›Paper›PMID 31453239›Full record

ArticleJournal of biological methods2018

Streamlined preparation of genomic DNA in agarose plugs for pulsed-field gel electrophoresis.

Linda Hicks, Charlotte M van der Graaf, Jacob Childress, Emily Cook, Karen Schmidt, Frank Rosenzweig, Eugene Kroll

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biological methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Linda HicksDivision of Biological Sciences, University of Montana, Missoula, MT 59812, USA.
Charlotte M van der GraafDivision of Biological Sciences, University of Montana, Missoula, MT 59812, USA.
Jacob ChildressDivision of Biological Sciences, University of Montana, Missoula, MT 59812, USA.
Emily CookDivision of Biological Sciences, University of Montana, Missoula, MT 59812, USA.
Karen SchmidtDivision of Biological Sciences, University of Montana, Missoula, MT 59812, USA.
Frank RosenzweigDivision of Biological Sciences, University of Montana, Missoula, MT 59812, USA.
Eugene KrollDivision of Biological Sciences, University of Montana, Missoula, MT 59812, USA.
University of Montana · USGeorgia Institute of Technology · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genome analysis using pulsed-field gel electrophoresis (PFGE) has been used in applications ranging from typing bacterial strains to radiobiology to cancer research. While methods for running PFGE have been significantly improved since its invention, the method for preparing chromosomal DNA itself has remained essentially unchanged. This limits the applicability of PFGE, especially when analyses require many samples. We have streamlined sample preparation for routine applications of PFGE through the use of deep-well 48-well plates. Besides saving time, our protocol has the added advantage of reducing the volume of expensive reagents. Our improved protocol enables us to reduce throughput time and simplify the procedure, facilitating wider application of PFGE-based analyses in the laboratory.

Indexed as

chromosomal DNA preparationdeep-well 48-well plateDNA plugsgenomic fingerprintingkaryotypingpulsed-field gel electrophoresis

Identifiers

PMID31453239
PMCPMC6706164
OpenAlexW2791217675

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.