ArticleJournal of biological methods2017
IL-6-HaloTag
Article in Journal of biological methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- New insights into the docking mechanism of vanillin on three mammalian olfactory receptors via a new statistical physics model.Scientific reports · 2025Article
- Roles of sensory receptors in non-sensory organs: the kidney and beyond.Nature reviews. Nephrology · 2025Review
- Antagonists Enhance Cell-Surface Expression of Mammalian Odorant Receptors.International journal of molecular sciences · 2025Article
- Article
- Petrol Note in Riesling - 1,1,6-Trimethyl-1,2-dihydronaphthalene (TDN) Selectively Activates Human Odorant Receptor OR8H1.Journal of agricultural and food chemistry · 2024Article
- M2OR: a database of olfactory receptor-odorant pairs for understanding the molecular mechanisms of olfaction.Nucleic acids research · 2024Article
- Enhancement of transcription efficiency by TAR-Tat system increases the functional expression of human olfactory receptors.PloS one · 2024Article
- Engineered odorant receptors illuminate structural principles of odor discrimination.bioRxiv : the preprint server for biology · 2023Article
- Heterodimerization of Chemoreceptors TAS1R3 and mGluInternational journal of molecular sciences · 2023Article
- Human Olfactory Receptor Sensor for Odor Reconstitution.Sensors (Basel, Switzerland) · 2023Review
- Modeling the Orthosteric Binding Site of the G Protein-Coupled Odorant Receptor OR5K1.Journal of chemical information and modeling · 2023Article
- The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit flyiScience · 2022Article
- Hot Spot Mutagenesis Improves the Functional Expression of Unique Mammalian Odorant Receptors.International journal of molecular sciences · 2021Article
- An evolutionary conserved olfactory receptor for foodborne and semiochemical alkylpyrazines.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Conserved C-terminal motifs in odorant receptors instruct their cell surface expression and cAMP signaling.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Structural instability and divergence from conserved residues underlie intracellular retention of mammalian odorant receptors.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase.Journal of visualized experiments : JoVE · 2019Article
- A bi-functional IL-6-HaloTagJournal of biological methods · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assignment of cognate odorant/agonist pairs is a prerequisite for an understanding of odorant coding at the receptor level. However, the identification of new ligands for odorant receptors (ORs) in cell-based assays has been challenging, due to their individual and rather sub-optimal plasma membrane expression, as compared with other G protein-coupled receptors. Accessory proteins, such as the chaperone RTP1S, or Ric8b, have improved the surface expression of at least a portion of ORs. Typically, recombinant ORs carry N-terminal tags, which proved helpful for their functional membrane expression. The most common tag is the 'Rho-tag', representing an N-terminal part of rhodopsin, but also 'Lucy-' or 'Flag-tag' extensions have been described. Here, we used a bi-functional N-terminal tag, called 'interleukin 6 (IL-6)-HaloTag
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.