ArticleJournal of biological methods2017
β-mercaptoethanol assists efficient construction of sperm bacterial artificial chromosome library.
Article in Journal of biological methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 2 citations in OpenAlex.
- Variant ribosomal DNA is essential for female differentiation in zebrafish.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- The admixed brushtail possum genome reveals invasion history in New Zealand and novel imprinted genes.Nature communications · 2023Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial artificial chromosome (BAC) library plays a critical role in the strategic research in genomics. Sperm is known as a good source for BAC library construction. However, preparation of intact DNA from the highly condensed sperm nuclei is not easy. Here we developed and validated an efficient DNA extraction strategy for BAC library construction from sperm embedded in agarose plugs. The protocol used a combination of lauroylsarcosine, proteinase K and β-mercaptoethanol (a reducing agent of nucleus) In comparison with the normal protocol without reducing agents, β-mercaptoethanol released high-molecular-weight DNA from the protamines which permit DNA to be packed very densely within the spermatozoan nucleus, without damaging DNA. Extracted DNA by this method was readily digested by restriction enzymes and ideal for BAC library construction.
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Registered trials
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