Evidence map›Paper›PMID 31453209›Full record

ArticleJournal of biological methods2016

Assessing bioenergetic functions from isolated mitochondria in Drosophila melanogaster.

Wen C Aw, Rijan Bajracharya, Samuel G Towarnicki, J William O Ballard

Abstract read
In one paragraph

Article in Journal of biological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Wen C AwSchool of Biotechnology and Biomolecular Science, University of New South Wales, Sydney 2052, Australia.
Rijan BajracharyaSchool of Biotechnology and Biomolecular Science, University of New South Wales, Sydney 2052, Australia.
Samuel G TowarnickiSchool of Biotechnology and Biomolecular Science, University of New South Wales, Sydney 2052, Australia.
J William O BallardSchool of Biotechnology and Biomolecular Science, University of New South Wales, Sydney 2052, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mitochondria are involved in generating more than 90 percent of cellular energy and are responsible for many cellular processes such as metabolism, cell signalling, apoptosis and ageing. Currently, there are a number of different experimental approaches employed to measure mitochondrial health and function. Here, we demonstrate a novel approach that quantifies substrate induced mitochondrial respiration from Drosophila. This protocol is optimized for mitochondria isolated from third instar larvae, and can also be used for mitochondria isolated from adult thoraces. This procedure outlines how to perform high throughput and high resolution mitochondria specific measurements for state II, state III, state IV

Indexed as

bioenergeticscomplex IDrosophilalarvaemitochondrial isolationSeahorse XF

Identifiers

PMID31453209
PMCPMC6706135

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.