ArticleJournal of biological methods2016
A comparison of strategies for immortalizing mouse embryonic fibroblasts.
Article in Journal of biological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Caveolin 1 is a conserved host factor mediating Bacillus cereus hemolysin BL toxin cytolytic toxicity.PLoS biology · 2026Article
- In vivo and in vitro analysis of functional effects of the SDHD H50R variant.Endocrine-related cancer · 2025Article
- Characterization and neurogenic responses of primary and immortalized Müller glia.Frontiers in cell and developmental biology · 2025Article
- Transcriptome Profiling of Mouse Embryonic Fibroblast Spontaneous Immortalization: A Comparative Analysis.International journal of molecular sciences · 2024Article
- Generation and characterization of human Fetal membrane and Decidual cell lines for reproductive biology experiments†.Biology of reproduction · 2022Article
- Mouse Embryonic Fibroblast Adipogenic Potential: A Comprehensive Transcriptome Analysis.Adipocyte · 2021Article
- Ras GEF Mouse Models for the Analysis of Ras Biology and Signaling.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Hepatocyte-like cells generated by direct reprogramming from murine somatic cells can repopulate decellularized livers.Biotechnology and bioengineering · 2018Article
- Hair follicle-derived mesenchymal cells support undifferentiated growth of embryonic stem cells.Experimental and therapeutic medicine · 2017Article
- Fibroblasts as an experimental model system for the study of comparative physiology.Comparative biochemistry and physiology. Part B, Biochemistry & molecular biologyArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genetically amenable mouse model has led to a large collection of genetically defined lines from which mouse embryonic fibroblasts (MEFs) have been derived. Despite their widespread use, MEFs are time consuming to generate and have a limited lifespan. Immortalizing primary MEFs with the desired genetic manipulations greatly reduces culture maintenance time, enables the generation of near limitless amounts of protein lysate, and facilitates biological replicates during experimentation. In this work, we have evaluated several approaches for MEF immortalization. When cultivated at 3% O
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Registered trials
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