ArticleJournal of advanced veterinary and animal research2019
Preservation of semen from Kintamani Bali dogs by freezing method.
Article in Journal of advanced veterinary and animal research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 18 citations in OpenAlex.
- Dose-dependent protective effects of p-coumaric acid on testicular ischemia/reperfusion injury in a mouse model of testicular torsion.Biochemistry and biophysics reports · 2026Article
- Naringin attenuates testicular ischemia/reperfusion injury in a mouse torsion/detorsion model via intraperitoneal administration.Pediatric surgery international · 2026Article
- Enhancement of pooled chilled semen quality in four Kintamani dogs through extenders enriched with different concentrations ofJournal of advanced veterinary and animal research · 2026Article
- Intratesticular copper oxide nanoparticles induce dose-dependent chemical castration in male mice.Scientific reports · 2025Article
- The synergistic activity of fisetin on quercetin improves testicular recover in ischemia-reperfusion injury in rats.Scientific reports · 2025Article
- Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology.BMC veterinary research · 2024Article
- Protective effects of Mito-TEMPO on ischemia-reperfusion injury in a mouse testicular torsion and detorsion model.Veterinary research forum : an international quarterly journal · 2024Article
- Evaluation of Chilled Dog Semen Extended With Sperm Activator.Frontiers in veterinary science · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo explore the effect of glycerol at different concentrations using different extenders on DNA fragmentation and motility of frozen-thawed Kintamani Bali dog spermatozoa. MATERIALS AND
methodsSample was collected from four mature Kintamani Bali dogs. Each ejaculate was prepared for cryopreservation with two different semen extenders; egg yolk Tris extender and coconut water-based extender. For each extender, three different glycerol concentrations were used; 4%, 6%, and 8%. Each of the six aliquots was loaded into 0.5 ml cryotube, placed on a styrofoam box 5 cm over liquid nitrogen for 10 min, and immersed in liquid nitrogen up to 8 min. Then, the frozen cryotubes were transferred into liquid nitrogen container. The cryotubes were thawed in a water bath at 38.5°C for 120 sec. After equilibration and thawing, each sample was assessed for motility parameters and for DNA fragmentation.
resultsThe addition of 6% glycerol to extenders revealed the most effective addition of glycerol on motility and sperm DNA fragmentation after equilibrium and post-thawing.
conclusionIt is concluded that both extenders with the addition of 6% glycerol are safe to be used as an extender in Kintamani Bali dog semen preservation, and DNA fragmentation of Kintamani Bali dog spermatozoa was not influenced by the freezing procedure.
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