ArticleBiomedical optics express2019
Volumetric stimulated Raman scattering imaging of cleared tissues towards three-dimensional chemical histopathology.
Article in Biomedical optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Next-generation kidney tissue analysis - spatial omics and digital pathology.Nature reviews. Nephrology · 2026Review
- AI-Assisted Coherent Raman Scattering Microscopy for Clinical Translation.Chemical & biomedical imaging · 2026Review
- Uses of coherent Raman scattering microscopy in neuroscience.Frontiers in neuroscience · 2025Review
- Revealing the MRI-Contrast in Optically Cleared Brains.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Virtual formalin-fixed and paraffin-embedded staining of fresh brain tissue via stimulated Raman CycleGAN model.Science advances · 2024Article
- Beyond 2D: A scalable and highly sensitive method for a comprehensive 3D analysis of kidney biopsy tissue.PNAS nexus · 2024Article
- Profiling single cancer cell metabolism via high-content SRS imaging with chemical sparsity.Science advances · 2023Article
- Label-Free Cytometric Evaluation of Mitosis via Stimulated Raman Scattering Microscopy and Spectral Phasor Analysis.Analytical chemistry · 2023Article
- Label-free structural and functional volumetric imaging by dual-modality optical-Raman projection tomography.Science advances · 2023Article
- Investigation of the Spatial Generation of Stimulated Raman Scattering Using Computer Simulation and Experimentation.Applied spectroscopy · 2022Article
- Tilt-angle stimulated Raman projection tomography.Optics express · 2022Article
- Dissecting lipid droplet biology with coherent Raman scattering microscopy.Journal of cell science · 2022Review
- Raman Scattering-Based Biosensing: New Prospects and Opportunities.Biosensors · 2021Review
- Multi-color stimulated Raman scattering with a frame-to-frame wavelength-tunable fiber-based light source.Biomedical optics express · 2021Article
- Coherent Raman scattering imaging with a near-infrared achromatic metalens.APL photonics · 2021Article
- Advances in stimulated Raman scattering imaging for tissues and animals.Quantitative imaging in medicine and surgery · 2021Review
- Tissue clearing technique: Recent progress and biomedical applications.Journal of anatomy · 2021Review
- Second harmonic generation imaging of collagen scaffolds within the alveolar ducts of healthy and emphysematous mouse lungs.Histochemistry and cell biology · 2021Article
- Volumetric chemical imaging in vivo by a remote-focusing stimulated Raman scattering microscope.Optics express · 2020Article
- Self-organizing pattern of subpleural alveolar ducts.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Thin tissue slice based histology has been used as a gold standard for disease diagnosis since over a hundred years ago. However, histopathological evaluation on two-dimensional slides suffers from large variations due to limited sampling. To improve the diagnostic accuracy, three-dimensional (3D) histology is performed through serial sectioning, staining, imaging and reconstruction of individual slices, which is highly time-consuming and labor intensive. We developed a volumetric stimulated Raman scattering (SRS) imaging method, which provides histology-like information in 3D context without the need for staining with dyes. Using a small molecule clearing agent, formamide, we performed tissue clearing within 30 min and achieved an imaging depth up to 500 µm in highly scattered tissues, including brain, kidney, liver and lung. Through a two-color SRS imaging scheme, we obtained histology-like images in cleared brain tissue slices. Our method has the potential for 3D tissue histopathology to improve the accuracy of histopathological examination.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.