ArticleMethods in molecular biology (Clifton, N.J.)2020
Two- and Three-Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium.
Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Review
- Apical-out bovine intestinal organoids as an infection model forCurrent research in parasitology & vector-borne diseases · 2025Article
- Rapid review: Recent advances inCurrent research in parasitology & vector-borne diseases · 2025Review
- Comparison of in vitro growth characteristics of Cryptosporidium hominis (IdA15G1) and Cryptosporidium parvum (Iowa-IIaA17G2R1 and IIaA18G3R1).Parasitology research · 2023Article
- Addressing Key Questions in Organoid Models: Who, Where, How, and Why?International journal of molecular sciences · 2023Review
- ExploringFrontiers in cellular and infection microbiology · 2023Review
- Differential Response to the Course of Cryptosporidium parvum Infection and Its Impact on Epithelial Integrity in Differentiated versus Undifferentiated Human Intestinal Enteroids.Infection and immunity · 2022Article
- Fully resolved assembly of Cryptosporidium parvum.GigaScience · 2022Article
- Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts.Journal of visualized experiments : JoVE · 2021Article
- Review
- Intestinal organoid/enteroid-based models for Cryptosporidium.Current opinion in microbiology · 2020Review
- Research in a time of enteroids and organoids: how the human gut model has transformed the study of enteric bacterial pathogens.Gut microbes · 2020Review
- Article
- A Conditional Protein Degradation System To Study Essential Gene Function in Cryptosporidium parvum.mBio · 2020Article
- Tools for probing host-bacteria interactions in the gut microenvironment: From molecular to cellular levels.Bioorganic & medicinal chemistry letters · 2020Review
- Analysis of Long Non-Coding RNA inFrontiers in cellular and infection microbiology · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Conventional cell cultures utilizing transformed or immortalized cell lines or primary human epithelial cells have played a fundamental role in furthering our understanding of Cryptosporidium infection. However, they remain inadequate with respect to their inability to emulate in vivo conditions, support long-term growth, and complete the life cycle of the parasite. Previously, we developed a 3D silk scaffold-based model using transformed human intestinal epithelial cells (IECs). This model supported C. parvum infection for up to 2 weeks and resulted in completion of the life cycle of the parasite. However, transformed IECs are not representative of primary human IEC.Human intestinal enteroids (HIEs) are cultures derived from crypts that contain Lgr5
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.