ArticleNature methods2019
Transmission-mode MALDI-2 mass spectrometry imaging of cells and tissues at subcellular resolution.
Article in Nature methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 192 papers, 1 of them a synthesis that pooled it.
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Who cites it
192 citing papers in PubMed, 1 synthesis or guideline pooled it, 410 citations in OpenAlex.
- Mass spectrometry-based human spatial omics: fundamentals, innovations, and applications.Journal of biomedical science · 2026Pooled it
- Visualizing Metabolism: Lipid-Based Raman Nanoreporters as an Enabling Platform for Metabolic Imaging and Precision Medicine.ACS nano medicine · 2026Review
- MALDI mass spectrometry imaging across spatial scales in toxicology: tissue evidence, cellular heterogeneity, and emerging single-cell opportunities.Analytical and bioanalytical chemistry · 2026Review
- LC-MS/MS and high-spatial single-cell MSI reveal selective downregulation of PC(16:0_16:0) and PC(18:0_18:1) due to mPGES-1 knockdown in hormone-resistant prostate cancer cell line.Analytical and bioanalytical chemistry · 2026Article
- RamanOmics decodes the spatial vibrational-molecular architecture of senescence in aging and repair.Nature aging · 2026Article
- Single-cell mass spectrometry imaging: platform advances for multimodal spatial omics.Analytical and bioanalytical chemistry · 2026Review
- Tissue expansion mass spectrometry imaging (TEMI) for high-spatial-resolution multiomics molecular mapping.Nature protocols · 2026Review
- Beyond the Fixed Charge: A New On-Tissue Derivatisation Strategy Employing Photoionisable Chromophores for Mass Spectrometry Imaging of Amino-Containing Metabolites.Angewandte Chemie (International ed. in English) · 2026Article
- Insights From Spatiality: Accelerating Pharmaceutical Research Using Mass Spectrometry Imaging.Medicinal research reviews · 2026Review
- Enhancing IR-MALDESI MSI Spatial Resolution Through Beam Constriction With a Ring-Actuated Iris.Rapid communications in mass spectrometry : RCM · 2026Article
- Isomer-Resolved Mass Spectrometry Imaging Reveals Regional and Age-Dependent Glycolipid Metabolism in Rat Brain.ACS central science · 2026Article
- Computational Mass Spectrometry Imaging in the Era of AI.Chemical reviews · 2026Review
- Sequential MALDI and Nano-DESI MS Imaging of Lipids and Proteins on Stretched Tissue Samples.Analytical chemistry · 2026Article
- Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.Analytical chemistry · 2026Article
- Automatic Coregistration of High-Resolution MALDI-MSI and Raman Imaging Applied to Cardiac Tissue of Fabry Disease Mouse Models.Analytical chemistry · 2026Article
- Development of a Tapping-Mode Scanning Probe Electrospray Ionization Platform for High-Sensitivity and Long-Term Stability in Single-Cell Mass Spectrometry Imaging of Tissue.Analytical chemistry · 2026Article
- Dissecting human organ development using spatial technologies.Developmental cell · 2026Review
- Enhancing the Sensitivity of Mass Spectrometry Imaging through Spatial Signal Averaging.Journal of the American Society for Mass Spectrometry · 2026Article
- Feature Down-Selection to Improve Supervised Classification by Machine Learning on Mass Spectrometry Imaging Data.Molecules (Basel, Switzerland) · 2026Article
- SPOTTER: Automated Tissue-Barcoding Platform for Spatial Proteomics and Phosphoproteomics.bioRxiv : the preprint server for biology · 2026Article
132 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption-ionization mass spectrometry imaging in transmission-mode geometry (t-MALDI-MSI) can provide molecular information with a pixel size of 1 µm and smaller, which makes this label-free method highly interesting for characterizing the chemical composition of tissues and cells on a (sub)cellular level. However, a major hindrance for wider use of the technology is the reduced ion abundance at small pixel sizes. Here we mitigate this problem by use of laser-induced post-ionization (MALDI-2) and by adapting a t-MALDI-2 ion source to an Orbitrap mass analyzer. We demonstrate the crucial sensitivity and accuracy boosts that are achieved with this combination by visualizing the distribution of numerous phospho- and glycolipids in mouse cerebellum and kidney slices, and in cultured Vero B4 cells. With brain tissue, a pixel size of 600 nm was achieved. Our method could constitute a valuable new tool for research in cell biology and biomedicine.
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